Isotope Labeling Methods for Relaxation Measurements

  • Patrik LundströmEmail author
  • Alexandra Ahlner
  • Annica Theresia Blissing
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 992)


Nuclear magnetic spin relaxation has emerged as a powerful technique for probing molecular dynamics. Not only is it possible to use it for determination of time constant(s) for molecular reorientation but it can also be used to characterize internal motions on time scales from picoseconds to seconds. Traditionally, uniformly 15N labeled samples have been used for these experiments but it is clear that this limits the applications. For instance, sensitivity for large systems is dramatically increased if dynamics is probed at methyl groups and structural characterization of low-populated states requires measurements on 13Cα, 13Cβ or 13CO or 1Hα. Unfortunately, homonuclear scalar couplings may lead to artifacts in the latter types of experiments and selective isotopic labeling schemes that only label the desired position are necessary. Both selective and uniform labeling schemes for measurements of relaxation rates for a large number of positions in proteins are discussed in this chapter.


Pentose Phosphate Pathway Label Scheme Relaxation Experiment Residue Type Chemical Shift Anisotropy 
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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Patrik Lundström
    • 1
    Email author
  • Alexandra Ahlner
    • 1
  • Annica Theresia Blissing
    • 1
  1. 1.Division of Molecular Biotechnology, Department of Physics, Chemistry and BiologyLinköping UniversityLinköpingSweden

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