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Isotope labeling in Biomolecular NMR pp 35–62Cite as

Isotope Labeling for Solution and Solid-State NMR Spectroscopy of Membrane Proteins

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 992))

Abstract

In this chapter, we summarize the isotopic labeling strategies used to obtain high-quality solution and solid-state NMR spectra of biological samples, with emphasis on integral membrane proteins (IMPs). While solution NMR is used to study IMPs under fast tumbling conditions, such as in the presence of detergent micelles or isotropic bicelles, solid-state NMR is used to study the structure and orientation of IMPs in lipid vesicles and bilayers. In spite of the tremendous progress in biomolecular NMR spectroscopy, the homogeneity and overall quality of the sample is still a substantial obstacle to overcome. Isotopic labeling is a major avenue to simplify overlapped spectra by either diluting the NMR active nuclei or allowing the resonances to be separated in multiple dimensions. In the following we will discuss isotopic labeling approaches that have been successfully used in the study of IMPs by solution and solid-state NMR spectroscopy.

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Abbreviations

IMP:

Integral Membrane Protein

SSNMR:

Solid-State NMR

O-SSNMR:

Oriented SSNMR

MAS-SSNMR:

Magic-Angle-Spinning SSNMR

PISEMA:

Polarization Inversion Spin Exchange at Magic Angle

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Authors and Affiliations

  1. Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN, 55455, USA

    Raffaello Verardi & Gianluigi Veglia

  2. Department of Chemistry, New York University, New York, NY, 10003, USA

    Nathaniel J. Traaseth

  3. Department of Chemistry, University of Minnesota, 6-155 Jackson Hall, 321 Church St SE, Minneapolis, MN, 55455, USA

    Larry R. Masterson, Vitaly V. Vostrikov & Gianluigi Veglia

Authors
  1. Raffaello Verardi
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  2. Nathaniel J. Traaseth
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  3. Larry R. Masterson
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  4. Vitaly V. Vostrikov
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  5. Gianluigi Veglia
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Correspondence to Gianluigi Veglia .

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  1. , NMR Research Centre, Indian Institute of Science, Bangalore, India

    Hanudatta S. Atreya

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Verardi, R., Traaseth, N.J., Masterson, L.R., Vostrikov, V.V., Veglia, G. (2012). Isotope Labeling for Solution and Solid-State NMR Spectroscopy of Membrane Proteins. In: Atreya, H. (eds) Isotope labeling in Biomolecular NMR. Advances in Experimental Medicine and Biology, vol 992. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4954-2_3

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