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Isotope Labeling for Solution and Solid-State NMR Spectroscopy of Membrane Proteins

  • Raffaello Verardi
  • Nathaniel J. Traaseth
  • Larry R. Masterson
  • Vitaly V. Vostrikov
  • Gianluigi VegliaEmail author
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 992)

Abstract

In this chapter, we summarize the isotopic labeling strategies used to obtain high-quality solution and solid-state NMR spectra of biological samples, with emphasis on integral membrane proteins (IMPs). While solution NMR is used to study IMPs under fast tumbling conditions, such as in the presence of detergent micelles or isotropic bicelles, solid-state NMR is used to study the structure and orientation of IMPs in lipid vesicles and bilayers. In spite of the tremendous progress in biomolecular NMR spectroscopy, the homogeneity and overall quality of the sample is still a substantial obstacle to overcome. Isotopic labeling is a major avenue to simplify overlapped spectra by either diluting the NMR active nuclei or allowing the resonances to be separated in multiple dimensions. In the following we will discuss isotopic labeling approaches that have been successfully used in the study of IMPs by solution and solid-state NMR spectroscopy.

Keywords

Nuclear Magnetic Resonance Isotopic Label Magic Angle Spin Nuclear Magnetic Resonance Study Maltose Binding Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

IMP

Integral Membrane Protein

SSNMR

Solid-State NMR

O-SSNMR

Oriented SSNMR

MAS-SSNMR

Magic-Angle-Spinning SSNMR

PISEMA

Polarization Inversion Spin Exchange at Magic Angle

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Raffaello Verardi
    • 1
  • Nathaniel J. Traaseth
    • 2
  • Larry R. Masterson
    • 3
  • Vitaly V. Vostrikov
    • 3
  • Gianluigi Veglia
    • 1
    • 3
    Email author
  1. 1.Department of Biochemistry, Molecular Biology, and BiophysicsUniversity of MinnesotaMinneapolisUSA
  2. 2.Department of ChemistryNew York UniversityNew YorkUSA
  3. 3.Department of ChemistryUniversity of MinnesotaMinneapolisUSA

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