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GPCR Signalling Complexes – Synthesis, Assembly, Trafficking and Specificity pp 155–180Cite as

Synthesis and Assembly of G Protein βγ Dimers: Comparison of In Vitro and In Vivo Studies

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Part of the book series: Subcellular Biochemistry ((SCBI,volume 63))

Abstract

The heterotrimeric GTP-binding proteins (G proteins) are the canonical cellular machinery used with the approximately 700 G protein-coupled receptors (GPCRs) in the human genome to transduce extracellular signals across the plasma membrane. The synthesis of the constituent G protein subunits, and their assembly into Gβγ dimers and G protein heterotrimers, determines the signaling repertoire for G-protein/GPCR signaling in cells. These synthesis/assembly ­processes are intimately related to two other overlapping events in the intricate pathway leading to formation of G protein signaling complexes, posttranslational modification and intracellular trafficking of G proteins. The assembly of the Gβγ dimer is a complex process involving multiple accessory proteins and organelles. The mechanisms involved are becoming increasingly appreciated, but are still incompletely understood. In vitro and in vivo (cellular) studies provide different perspectives of these processes, and a comparison of them can provide insight into both our current level of understanding and directions to be taken in future investigations.

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Authors and Affiliations

  1. Department of Cell and Molecular Pharmacology, Medical University of South Carolina, Charleston, SC, 29425, USA

    Jane Dingus & John D. Hildebrandt

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  1. Jane Dingus
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  2. John D. Hildebrandt
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Correspondence to John D. Hildebrandt .

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Editors and Affiliations

  1. Faculty of Medicine, Pharmacology, Dalhousie University, College Street 5968, Halifax, B3H 4R2, Nova Scotia, Canada

    Denis J. Dupré

  2. , Pharmacology and Therapeutics, McGill University, Promenade Sir William Osler 3655, Montreal, H3G 1Y6, Québec, Canada

    Terence E. Hébert

  3. Institut Cochin, Rue Méchain 22, Paris, 75014, France

    Ralf Jockers

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© 2012 Springer Science+Business Media Dordrecht

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Dingus, J., Hildebrandt, J.D. (2012). Synthesis and Assembly of G Protein βγ Dimers: Comparison of In Vitro and In Vivo Studies. In: Dupré, D., Hébert, T., Jockers, R. (eds) GPCR Signalling Complexes – Synthesis, Assembly, Trafficking and Specificity. Subcellular Biochemistry, vol 63. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4765-4_9

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