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Seed Development: OMICS Technologies toward Improvement of Seed Quality and Crop Yield pp 27–41Cite as

Proteomics Reveals A Potential Role of the Perisperm in Starch Remobilization During Sugarbeet Seed Germination

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Abstract

Sugarbeet is a crop of high economic importance because it is one of the two main sources of plant sugar, the other being sugarcane. The sugarbeet seeds have the peculiarity of containing at maturity a large starchy storage tissue, called the perisperm. In contrast to the well-documented cereal endosperm, the physiology of this perisperm is completely unknown. Here, we used proteomics of perisperm isolated either from dry mature or imbibed sugarbeet seeds to unravel the mechanisms of starch remobilization during germination. We also carried out a comparative proteomics analysis with the perisperm isolated from the dry mature sugarbeet seeds. We observed an accumulation of α-amylase in the perisperm isolated from imbibed whole seeds but not from the isolated imbibed perisperm alone, suggesting a role of the embryo in triggering the accumulation of this starch-mobilizing enzyme in the perisperm during germination. In this way, the mechanisms occurring in the sugarbeet seed perisperm during germination would appear to be similar to those documented for the endosperm of cereals. In contrast, an accumulation of β-amylase and α-glucosidase was observed in the isolated imbibed perisperm, suggesting that the embryo was not mandatory for induction of these enzymes in the perisperm during imbibition.

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Acknowledgment

This work was supported by the French Ministry of Research. We thank Juliane Meinhard, Andreas Menze, and Uwe Fischer for helpful discussions, and Emeline Leydier, Camille Bechetoille, and Bernadette Claus for skillful technical assistance.

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  1. CNRS/Bayer Crop Science Joint Laboratory (UMR5240), Bayer Crop Science, 14-20 rue Pierre Baizet, F-69263, Lyon cedex, France

    Claudette Job & Dominique Job

  2. Quintiles – Navigating the new health, Parcd d’nnovation, rue Jean Dominique Cassini, F-67404, Illkirch cedex, France

    Julie Catusse

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    Ganesh K. Agrawal

  2. , Biology, University of Tsukuba, Tennoudai 1-1-1, Tsukuba, 305-8572, Japan

    Randeep Rakwal

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Catusse, J., Job, C., Job, D. (2012). Proteomics Reveals A Potential Role of the Perisperm in Starch Remobilization During Sugarbeet Seed Germination. In: Agrawal, G., Rakwal, R. (eds) Seed Development: OMICS Technologies toward Improvement of Seed Quality and Crop Yield. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4749-4_2

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