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Digging Deeper into the Seed Proteome: Prefractionation of Total Proteins

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Seed Development: OMICS Technologies toward Improvement of Seed Quality and Crop Yield

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Abstract

Seeds are a common experimental system for many reasons. Among these: (i) they occupy a major niche in agriculture and human nutrition; (ii) they are a rich source of critical genetic information; and (iii) they are a near-ideal system for the study of phytohormone action or the transition from either dormancy or quiescence to active growth and development. One important component of all of these considerations is occurrence of the highly-abundant seed storage proteins (SSP). While on the one hand the high levels of proteins present in seeds make them attractive subjects, the SSP themselves are anathema to proteomics analyses. Without some sort of pretreatment removal of SSP, they will be virtually the only proteins identified in shotgun proteomics analyses. Here in, we describe and compare several methods commonly used to deplete samples of SSP, present the relatively recent application of combinatorial-ligand random-peptide libraries to seed proteomics studies, and speculate briefly on the short-term future.

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Authors and Affiliations

  1. USDA, Agricultural Research Service, Plant Genetics Research Unit, University of Missouri, 65211, Columbia, MO, USA

    Ján A. Miernyk & Mark L. Johnston

  2. Department of Biochemistry, University of Missouri, 65211, Columbia, MO, USA

    Ján A. Miernyk

  3. Interdisciplinary Plant Group, University of Missouri, 65211, Columbia, MO, USA

    Ján A. Miernyk

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  1. Ján A. Miernyk
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  2. Mark L. Johnston
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Correspondence to Ján A. Miernyk .

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Editors and Affiliations

  1. RLABB, Sanepa, Lalitpur, Nepal

    Ganesh K. Agrawal

  2. , Biology, University of Tsukuba, Tennoudai 1-1-1, Tsukuba, 305-8572, Japan

    Randeep Rakwal

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Miernyk, J.A., Johnston, M.L. (2012). Digging Deeper into the Seed Proteome: Prefractionation of Total Proteins. In: Agrawal, G., Rakwal, R. (eds) Seed Development: OMICS Technologies toward Improvement of Seed Quality and Crop Yield. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4749-4_14

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