Abstract
Barley hordoindoline genes (Hina and Hinb) are homologous to wheat puroindoline genes (Pina and Pinb). These genes are involved in grain hardness, which is an important quality characteristic for barley processing. However, the interspecific variation in the Hin genes in the genus Hordeum has not been studied in detail. We examined the variation in Hin genes and used it to infer the phylogenetic relationships between the genes found in two H. vulgare species and 10 wild relatives. Our results suggest that the Hinb gene duplicated during the early stages of speciation in Hordeum. We also identified novel variant forms of Hina and Hinb genes in the wild Hordeum species and preliminarily named them Hinc. The phylogenetic tree of Gsp-1, Hin, and Pin genes shown demonstrates that Hinc and Pinb-2v genes formed one cluster. Therefore, we considered that Hinc and Pinb-2v genes shared a common ancestral gene and were homologous to each other. We also studied the evolutional process of Gsp-1, Hin, and Pin genes. Our results suggested that Gsp-1 genes might be the most closely related to an ancestral gene on Ha locus.
*Presenting author, Yohei Terasawa
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Notes
- 1.
These lines were kindly provided by Dr. S. Taketa, Okayama University, Japan.
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Acknowledgments
This work was supported by the Bio-oriented Technology Research Advancement Institution. We express our sincere gratitude to Dr. Shin Taketa for providing material support.
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Terasawa, Y., Takata, K., Ikeda, T.M. (2013). The Distribution of the Hordoindoline Genes and Identification of Puroindoline b-2 Variant Gene Homologs in the Genus Hordeum . In: Zhang, G., Li, C., Liu, X. (eds) Advance in Barley Sciences. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4682-4_7
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DOI: https://doi.org/10.1007/978-94-007-4682-4_7
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