Abstract
A series of recombinant chromosome substitution lines (RCSLs) and a near-isogenic line on the isolated aluminum tolerance locus were developed from a cross between elite cultivar Haruna Nijo and aluminum-tolerant cultivar Murasakimochi. High-throughput single nucleotide polymorphism (SNP) genotyping has previously been developed for barley. The parents were genotyped with 1,448 unigene-derived SNPs. Of these 1,448 SNPs, 690 were polymorphic between Haruna Nijo and Murasakimochi, giving an overall polymorphism rate of 47.7%. These 690 SNPs were compared with the consensus map developed by the same marker system, and the numbers of markers per chromosome were as follows: 1H (78), 2H (101), 3H (120), 4H (97), 5H (113), 6H (94), and 7H (87). The SNP markers were well-distributed and reflecting the original distribution of consensus map. The SNPs used to genotype 83 BC3F3 (RCSLs). These data were used to create graphical genotypes for each line and thus estimate the location, extent, and total number of introgressions from Murasakimochi in the Haruna Nijo background. The selected 39 RCSLs sample most of the Murasakimochi genome.
Presenting author, Kazuhiro Sato
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Acknowledgments
We would like to thank Dr. Joe Deyong, Southern California Genotyping Consortium, Illumina BeadLab, UCLA for the OPA-SNP assay; Drs. Timothy J. Close and Prasanna Bhat, Department of Botany and Plant Sciences, University of California, Riverside for basecalling of OPA assay.
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© 2013 Zhejiang University Press and Springer Science+Business Media Dordrecht
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Sato, K., Ma, J. (2013). Development of Recombinant Chromosome Substitution Lines for Aluminum Tolerance in Barley. In: Zhang, G., Li, C., Liu, X. (eds) Advance in Barley Sciences. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4682-4_28
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DOI: https://doi.org/10.1007/978-94-007-4682-4_28
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