Abstract
Spot blotch, an economically important disease of both barley and wheat, is caused by Cochliobolus sativus (anamorph: Bipolaris sorokiniana). The disease has been reported in many regions of the world but is particularly severe on barley in the Upper Midwest region of the United States, adjacent areas of Canada, and northeastern Australia. Durable resistance has been attained in Midwest six-rowed malting cultivars for over 50 years and is derived from line NDB112. To identify loci conferring resistance to spot blotch, an association mapping approach was conducted in US breeding germplasm (3,840 lines) genotyped with 3,072 single-nucleotide polymorphism (SNP) markers. Three quantitative trait loci (QTL) Rcs-qtl-1H-11_10764, Rcs-qtl-3H-11_10565, and Rcs-qtl-7H-11_20162 conferring both seedling and adult resistance were identified. Each individual QTL only partially reduced the infection response (IR) (from 0 to 20%) at the seedling stage and severity (from 20 to 29%) at the adult plant stage. However, all three QTL together reduced the seedling IR and adult plant severity by 47 and 83%, respectively, and comprise the Midwest six-rowed durable resistance haplotype (MSDRH). The identified MSDRH will be valuable for marker-assisted selection of spot blotch resistance in breeding programs.
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References
Benjamini, Y., & Hochberg, Y. (1995). Controlling the false discovery rate: A practical and powerful approach to multiple testing. Journal of Royal Statistical Society, 57, 289–300.
Close, T. J., Bhat, P. R., Lonardi, S., Wu, Y., Rostoks, N., Ramsay, L., Druka, A., Stein, N., Svensson, J. T., Wanamaker, S., Bozdag, S., Roose, M. L., Moscou, M. J., Chao, S., Varshney, R. K., Szucs, P., Sato, K., Hayes, P. M., Matthews, D. E., Kleinhofs, A., Muehlbauer, G. J., DeYoung, J., Marshall, D. F., Madishetty, K., Fenton, R. D., Condamine, P., Graner, A., & Waugh, R. (2009). Development and implementation of high-throughput SNP genotyping in barley. BMC Genomics, 10, 582.
Fetch, T. G., & Steffenson, B. J. (1994). Identification of Cochliobolus sativus isolates expressing differential virulence on two-row barley genotypes from North Dakota. Canadian Journal of Plant Pathology, 16, 202–206.
Flint-Garcia, S. A., & Thornsberry, J. M. (2003). Structure of linkage disequilibrium in plants. Annual Review of Plant Biology, 54, 357–374.
Knight, N. L., Platz, G. J., Lehmensiek, A., & Sutherland, M. W. (2010). An investigation of genetic variation among Australian isolates of Bipolaris sorokiniana from different cereal tissues and comparison of their abilities to cause spot blotch on barley. Australasian Plant Pathology, 39, 207–216.
Mathre, D. E. (1997). Compendium of barley diseases (2nd ed.). St. Paul: The American Phytopathological Society Press.
Parisseaux, B., & Bernardo, R. (2004). In silico mapping of quantitative trait loci in maize. Theoretical and Applied Genetics, 109, 508–514.
Sprague, R. (1950). Diseases of cereals and grasses in North America: Fungi, except smuts and rusts. New York: Ronald Press.
Steffenson, B. J. (2000). Durable resistance to spot blotch and stem rust in barley. In Proceedings of the 8th International Barley Genetics Symposium, Glen Osmond (Barley Genetics VIII, Vol. I, pp. 39–44).
Steffenson, B. J., Hayes, P. M., & Kleinhofs, A. (1996). Genetics of seedling and adult plant resistance to net blotch (Pyrenophora teres f. teres) and spot blotch (Cochliobolus sativus) in barley. Theoretical and Applied Genetics, 92, 552–558.
Storey, J. D. (2002). A direct approach to false discovery rates. Journal of the Royal Statistical Society, 64, 479–498.
Valjavec-Gratian, M., & Steffenson, B. J. (1997). Pathotypes of Cochliobolus sativus on barley in North Dakota. Plant Disease, 81, 1275–1278.
Wilcoxson, R. D., Rasmusson, D. C., & Miles, M. R. (1990). Development of barley resistant to spot blotch and genetics of resistance. Plant Disease, 74, 207–210.
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© 2013 Zhejiang University Press and Springer Science+Business Media Dordrecht
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Zhou, H., Steffenson, B.J. (2013). Genome-Wide Association Mapping Reveals Genetic Architecture of Durable Spot Blotch Resistance in US Barley Breeding Germplasm. In: Zhang, G., Li, C., Liu, X. (eds) Advance in Barley Sciences. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4682-4_22
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DOI: https://doi.org/10.1007/978-94-007-4682-4_22
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