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Detection of Telomerase Activity: A New Strategy for Detecting Low Activity of Telomerase

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Telomere Territory and Cancer

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Abstract

For detecting telomerase activity, a PCR-based technique known as TRAP assay was introduced in 1994. Since then, by innovating complementary design many alterations were applied to improve the quality of detection and reducing the problems occurred during amplification and detection of the telomerase products. Especially, such complications could occur in samples having low quantities of telomerase enzyme and/or PCR inhibitors. A new strategy focusing on re-amplification of the products could help the investigators to determine the majority of telomerase-positive samples.

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Authors and Affiliations

  1. Medical Genetics Lab., Imam Hospital, Department of Genetics, Ardabil University of Medical Sciences, Ardabil, Iran

    S. Saied Hosseini-Asl

  2. Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Dr. P. Mehdipour

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  1. S. Saied Hosseini-Asl
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  2. Dr. P. Mehdipour
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Editors and Affiliations

  1. School Of Medicine, Tehran University of Medical Sciences, Department of Medical Genetics, Pour Sina Avenue, Tehran, 1417613151, Iran

    Parvin Mehdipour

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Saied Hosseini-Asl, S., Mehdipour, P. (2013). Detection of Telomerase Activity: A New Strategy for Detecting Low Activity of Telomerase. In: Mehdipour, P. (eds) Telomere Territory and Cancer. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4632-9_3

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