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Postnatal Hyperoxia Impairs Acute Oxygen Sensing of Rat Glomus Cells by Reduced Membrane Depolarization

  • Insook KimEmail author
  • David F. Donnelly
  • John L. Carroll
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 758)

Abstract

Previous work demonstrated that hyperoxia (30–60% O2) exposure in the post-natal period reduces the ventilatory response to acute hypoxia and this impairment may continue considerably beyond the period of hyperoxia exposure. Previous work from our laboratory demonstrated that 1–2 weeks of hyperoxia (60% O2) starting between P1 and P14: reduced the single chemoreceptor unit response to hypoxia, reduced the rise in glomus cell calcium caused by acute hypoxia and reduced hypoxia-induced catecholamine release (Donnelly 05, Donnelly 09). The present study asked whether the impairment extended to hypoxia-induced membrane depolarization, an earlier step in the transduction cascade. Perforated patch, whole-cell recordings were obtained from rat glomus cells exposed to hyperoxia from P0-P8 or P8-P15 and age-matched control groups. In both cases, hypoxia-induced membrane depolarization was significantly less in the hyperoxia treated groups compared to controls, while depolarization to 20 mM K+ was not significantly affected. Resting membrane potential and input resistance were also not different in the hyperoxia treated groups. Whole carotid body quantitative real time PCR showed that TASK-1, TASK-3 and L-type Ca2+ channel expression was significantly down-regulated at Hyper 8–15 compared to controls. We conclude that 1 week of postnatal hyperoxia during the early and late stage of CB maturation impairs organ function by affecting the coupling between hypoxia and glomus cell depolarization. This may be caused by altered expression of TASK1, TASK3 or L-type Ca2+ channel gene expression. We speculate that an identification of cellular changes caused by hyperoxia may yield unique insights to the mechanism of oxygen sensing by the carotid bodies.

Keywords

Carotid body Glomus cells Hyperoxia Membrane depolarization Gene expression qPCR Postnatal Impairment 

Notes

Acknowledgements

This study was supported by grants from National Institute of health (HL-073500 (DFD) and HL-05621 (JLC)).

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Insook Kim
    • 1
    Email author
  • David F. Donnelly
    • 2
  • John L. Carroll
    • 1
    • 3
  1. 1.Department of PediatricsACHRI, University of Arkansas for Medical SciencesLittle RockUSA
  2. 2.Department of PediatricsYale University, School of MedicineNew HavenUSA
  3. 3.Arkansas Children’s HospitalLittle RockUSA

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