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ATP Release from the Carotid Bodies of DBA/2J and A/J Inbred Mouse Strains

  • Pejmon Pashai
  • Eric W. Kostuk
  • Luis E. Pilchard
  • Machiko ShirahataEmail author
Conference paper
  • 3.5k Downloads
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 758)

Abstract

The purposes of this study were to: (1) establish an effective method to measure the release of ATP from the mouse carotid body (CB) and (2) determine the release of ATP from the CB of the DBA/2 J (high hypoxic responder) and A/J (low hypoxic responder) mouse in response to hypoxia and hypercapnia. An incubation chamber was constructed utilizing a Costar® Spin-X Centrifuge Tube Filter. The filter was coated with low melting point agarose to hold 4 CBs or 4 superior cervical ganglia (SCG). Hypoxia did not increase ATP release from the CB of either strain. ATP increased in response to a normoxic/hypercapnic challenge in the DBA/2 J’s CB but not in the A/J’s CB. ATP release from the SCG was affected by neither hypoxia nor hypercapnia in both strains. Thus, we have concluded: (1) we successfully established a chamber system to measure ATP released from the mouse CB; (2) ATP may not be an excitatory neurotransmitter in the CB of these mice under hypoxia; (3) ATP may be a neurotransmitter in the CB of the DBA/2 J mouse strain during hypercapnia.

Keywords

Carotid body Hypoxia Hyperoxia Neurotransmitter 

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Pejmon Pashai
    • 1
  • Eric W. Kostuk
    • 1
  • Luis E. Pilchard
    • 1
  • Machiko Shirahata
    • 1
    Email author
  1. 1.Division of Physiology, Department of Environmental Health Sciences, Bloomberg School of Public HealthJohns Hopkins UniversityBaltimoreUSA

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