Abstract
The purposes of this study were to: (1) establish an effective method to measure the release of ATP from the mouse carotid body (CB) and (2) determine the release of ATP from the CB of the DBA/2 J (high hypoxic responder) and A/J (low hypoxic responder) mouse in response to hypoxia and hypercapnia. An incubation chamber was constructed utilizing a Costar® Spin-X Centrifuge Tube Filter. The filter was coated with low melting point agarose to hold 4 CBs or 4 superior cervical ganglia (SCG). Hypoxia did not increase ATP release from the CB of either strain. ATP increased in response to a normoxic/hypercapnic challenge in the DBA/2 J’s CB but not in the A/J’s CB. ATP release from the SCG was affected by neither hypoxia nor hypercapnia in both strains. Thus, we have concluded: (1) we successfully established a chamber system to measure ATP released from the mouse CB; (2) ATP may not be an excitatory neurotransmitter in the CB of these mice under hypoxia; (3) ATP may be a neurotransmitter in the CB of the DBA/2 J mouse strain during hypercapnia.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Buttigieg J, Nurse CA (2004) Detection of hypoxia-evoked ATP release from chemoreceptor cells of the rat carotid body. Biochem Biophys Res Commun 322:82–87
Campen MJ, Tagaito Y, Li JG, Balbir A, Tankersley CG, Smith P, Schwartz A, O’Donnell CP (2004) Phenotypic variation in cardiovascular responses to acute hypoxic and hypercapnic exposure in mice. Physiol Genomics 20:15–20
Campen MJ, Tagaito Y, Jenkins TP, Balbir A, O’Donnell CP (2005) Heart rate variability responses to hypoxic and hypercapnic exposures in different mouse strains. J Appl Physiol 99:807–813
Fitzgerald RS, Shirahata M, Chang I, Kostuk E (2009) The impact of hypoxia and low glucose on the release of acetylcholine and ATP from the incubated cat carotid body. Brain Res 1270:39–44
He L, Chen J, Dinger B, Stensaas L, Fidone S (2006) Effect of chronic hypoxia on purinergic synaptic transmission in rat carotid body. J Appl Physiol 100:157–162
Omar SH (1993) Oxygen diffusion through gels employed for immobilization 1 in the absence of microorganisms. Appl Microbiol Biotechnol 40:1–6
Pichard L, Sgmabti F, Kostuk E, Pashai P, Fitzgerald RS, Shirahata M (2011) Murine carotid body responses to hypoxia: in vivo carotid sinus nerve recordings in the DBA/2J and A/J strains. FASEB 24:1026.19
Reyes EP, Fernández R, Larraín C, Zapata P (2007) Effects of combined cholinergic-purinergic block upon cat carotid body chemoreceptors in vitro. Respir Physiol Neurobiol 156:17–22
Rong WF, Gourine AV, Cockayne DA, Xiang ZH, Ford APDW, Spyer KM, Burnstock G (2003) Pivotal role of nucleotide P2X(2) receptor subunit of the ATP-gated ion channel mediating ventilatory responses to hypoxia. J Neurosci 23:11315–11321
Rubin AE, Polotsky VY, Balbir A, Krishnan JA, Schwartz AR, Smith PL, Fitzgerald RS, Tankersley CG, Shirahata M, O’Donnell CP (2003) Differences in sleep-induced hypoxia between a/J and DBA/2 J mouse strains. Am J Respir Crit Care Med 168:1520–1527
Santos PF, Caramelo OL, Carvalho AP, Duarte CB (1999) Characterization of ATP release from cultures enriched in cholinergic amacrine-like neurons. J Neurobiol 41:340–348
Tankersley CG, Fitzgerald RS, Kleeberger SR (1994) Differential control of ventilation among inbred strains of mice. Am J Physiol Regul Integr Comp Physiol 267:R1371–R1377
Yamaguchi S, Balbir A, Schofield B, Coram J, Tankersley CG, Fitzgerald RS, O’Donnell CP, Shirahata M (2003) Structural and functional differences of the carotid body between DBA/2 J and a/J strains of mice. J Appl Physiol 94:1536–1542
Yamaguchi S, Lande B, Kitajima T, Hori Y, Shirahata M (2004) Path clamp study of mouse glomus cells using whole carotid body. Neurosci Lett 357:155–157
Zhang M, Zhong H, Vollmer C, Nurse CA (2000) Co-release of ATP and ACh mediates hypoxic signalling at rat carotid body chemoreceptors. J Physiol 525:143–158
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer Science+Business Media Dordrecht
About this paper
Cite this paper
Pashai, P., Kostuk, E.W., Pilchard, L.E., Shirahata, M. (2012). ATP Release from the Carotid Bodies of DBA/2J and A/J Inbred Mouse Strains. In: Nurse, C., Gonzalez, C., Peers, C., Prabhakar, N. (eds) Arterial Chemoreception. Advances in Experimental Medicine and Biology, vol 758. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4584-1_38
Download citation
DOI: https://doi.org/10.1007/978-94-007-4584-1_38
Published:
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-007-4583-4
Online ISBN: 978-94-007-4584-1
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)