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Purinergic Modulation of Carotid Body Glomus Cell Hypoxia Response During Postnatal Maturation in Rats

  • John L. CarrollEmail author
  • Amit Agarwal
  • David F. Donnelly
  • Insook Kim
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 758)

Abstract

Carotid body (CB) glomus cells respond to hypoxia by releasing neurotransmitters, such as ATP, which are believed to stimulate excitatory receptors on apposed nerve endings of the carotid sinus nerves as well as bind to autoreceptors on the glomus cell membrane to modulate response magnitude. The CB response to hypoxia is small at birth and increases during postnatal maturation in mammals. As ATP has been shown to inhibit the glomus cell response to hypoxia via an autoreceptor mechanism, we hypothesized that ATP-mediated inhibition may vary with age and play a role in postnatal development of the hypoxia response magnitude. The effects of ATP on CB glomus cell intracellular calcium ([Ca2+]i) responses to hypoxia were studied at two ages, P0-1 and P14-18. The inhibitory effect of ATP or a stable ATP analog on the glomus cell response to hypoxia was greater in newborn rats compared to the more mature age group. Use of selective P2Y receptor agonists and antagonists suggests that the inhibitory effect of ATP on the glomus cell [Ca2+]i response to hypoxia may be mediated by a P2Y12 receptor. Thus, developmental changes in ATP-mediated glomus cell inhibition may play a role in carotid chemoreceptor postnatal maturation.

Keywords

Carotid body Glomus cells Hypoxia ATP Postnatal Development Maturation Purinergic receptor 

Notes

Acknowledgements

This study was supported by grants from National Institutes of Health (HL-05621 NIH RO1) and Arkansas Biosciences Institute (ABI).

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • John L. Carroll
    • 1
    Email author
  • Amit Agarwal
    • 1
  • David F. Donnelly
    • 2
  • Insook Kim
    • 1
  1. 1.Division of Pediatric Pulmonary Medicine, Department of PediatricsUniversity of Arkansas for Medical SciencesLittle RockUSA
  2. 2.Dept of PediatricsYale University School of MedicineNew HavenUSA

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