Abstract
In the zebrafish, O2-chemoreceptive neuroepithelial cells (NECs) of the gill arches detect changes in PO2 and are believed to initiate cardiorespiratory responses to hypoxia. Goldfish have gill NECs of similar morphology and innervation, yet these animals are naturally tolerant to prolonged periods of anoxia. Whole-cell, voltage-clamp experiments indicated that goldfish NECs express a variety of membrane ion channels, including background and Ca2+-activated K+ channels. Our initial studies suggest that goldfish NECs do not respond equally to hypoxia (N2, 25 mmHg) as do those of zebrafish; however, current-clamp recordings indicated that anoxia produced membrane depolarization. In addition, we found that cyanide, which mimics hypoxia, depolarized NECs. Our goal is to further characterize these membrane conductances and determine their potential contribution to O2 sensing in NECs of the anoxia-tolerant goldfish.
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References
Buckler KJ (1997) A novel oxygen-sensitive potassium current in rat carotid body type I cells. J Physiol 498(Pt 3):649–662
Burggren WW (1982) “Air gulping” improves blood oxygen transport during aquatic hypoxia in the goldfish Carassius Auratus. Physiol Zool 55:327
Burleson ML, Mercer SE, Will-Blaszczak MA (2006) Isolation and characterization of putative O2 chemoreceptor cells from the gills of channel catfish (Ictalurus punctatus). Brain Res 1092:100–107
Bushnell PG, Steffensen JF, Johansen K (1984) Oxygen consumption and swimming performance in hypoxia-acclimated rainbow trout Salmo gairdneri. J Exp Biol 113:225–235
Campanucci VA, Fearon IM, Nurse CA (2003) A novel O2-sensing mechanism in rat glossopharyngeal neurones mediated by a halothane-inhibitable background K + conductance. J Physiol 548:731–743
Guia A, Wan X, Courtemanche M, Leblanc N (1999) Local Ca2+ entry through L-type Ca2+ channels activates Ca2 + −dependent K + channels in rabbit myocytes. Circ Res 84:1032–1042
Herrera GM, Etherton B, Nausch B, Nelson MT (2005) Negative feedback regulation of nerve-mediated contractions by KCa channels in mouse urinary bladder smooth muscle. Am J Physiol Regul Integr Comp Physiol 289:R402–R409
Jonz MG, Nurse CA (2003) Neuroepithelial cells and associated innervation of the zebrafish gill: a confocal immunofluorescence study. J Comp Neurol 461:1–17
Jonz MG, Fearon IM, Nurse CA (2004) Neuroepithelial oxygen chemoreceptors of the zebrafish gill. J Physiol 560:737–752
Lopez-Lopez JR, Gonzalez C, Perez-Garcia MT (1997) Properties of ionic currents from isolated adult rat carotid body chemoreceptor cells: effect of hypoxia. J Physiol 499(Pt 2):429–441
Mandic M, Todgham AE, Richards JG (2009) Mechanisms and evolution of hypoxia tolerance in fish. Proc R Soc B 276:735–744
O’Kelly I, Stephens RH, Peers C, Kemp PJ (1999) Potential identification of the O2-sensitive K + current in a human neuroepithelial body-derived cell line. Am J Physiol 276:L96–L104
Peers C, Wyatt CN (2007) The role of maxiK channels in carotid body chemotransduction. Respir Physiol Neurobiol 157:75–82
Perry SF, Jonz MG, Gilmour KM (2009) Oxygen sensing and the hypoxic ventilatory response. In: Richards JG, Farrell AP, Brauner CJ (eds) Hypoxia. Elsevier, New York, p 194
Saltys HA, Jonz MG, Nurse CA (2006) Comparative study of gill neuroepithelial cells and their innervation in teleosts and xenopus tadpoles. Cell Tissue Res 323:1–10
Sundin L, Nilsson S (2002) Branchial Innervation. J Exp Zool 293:232–248
Wyatt CN, Buckler KJ (2004) The effect of mitochondrial inhibitors on membrane currents in isolated neonatal rat carotid body type I cells. J Physiol 556(1):175–191
Youngson C, Nurse C, Yeger H, Cutz E (1993) Oxygen sensing in airway chemoreceptors. Nature 365:153–155
Zhang L, Nurse CA, Jonz MG, Wood CM (2011) Ammonia sensing by neuroepithelial cells and ventilatory responses to ammonia in rainbow trout. J Exp Biol 214:2678–2689
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© 2012 Springer Science+Business Media Dordrecht
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Zachar, P.C., Jonz, M.G. (2012). Oxygen Sensitivity of Gill Neuroepithelial Cells in the Anoxia-Tolerant Goldfish. In: Nurse, C., Gonzalez, C., Peers, C., Prabhakar, N. (eds) Arterial Chemoreception. Advances in Experimental Medicine and Biology, vol 758. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4584-1_23
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DOI: https://doi.org/10.1007/978-94-007-4584-1_23
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