Acute Hypoxia Does Not Influence Intracellular pH in Isolated Rat Carotid Body Type I Cells

  • Ryan L. Shapiro
  • Barbara L. Barr
  • Robert W. Putnam
  • Christopher N. WyattEmail author
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 758)


In order to interpret data obtained from isolated Type I cells during hypoxia it is critical to know whether parameters such as intracellular pH (pHi) vary during this challenge. Multiple studies have attempted to address this issue but data are contradictory and methods poorly defined (Wilding et al. 1993; Pang and Eyzaguirre 1993). Wilding et al. used BCECF loaded, isolated Type I cells and demonstrated that ‘hypoxia’ did not cause a change in pHi. However, solutions in these experiments were gassed with 2% oxygen and recording chamber PO2 was not reported. Conversely, Pang and Eyzaguirre used pH-sensitive microelectrodes and demonstrated that single isolated Type I cells did acidify during a hypoxic challenge whereas clusters of Type I cells did not.


Carotid body Hypoxia Intracellular pH 



RLS was privately funded by R. M. Johnson MD, T. Perry MD and R. Laughlin MD. CNW was funded by NIH 1RO1HL091836


  1. Buckler KJ, Vaughan-Jones RJ (1990) Application of a new pH-sensitive fluoroprobe (carboxy-SNARF-1) for intracellular pH measurement in small, isolated cells. Pflügers Archiv 417(2):234–239PubMedCrossRefGoogle Scholar
  2. Burlon DC, Jordan HL, Wyatt CN (2009) Presynaptic regulation of isolated neonatal rat carotid body type I cells by histamine. Respir Physiol Neurobiol 168:218–223PubMedCrossRefGoogle Scholar
  3. Garcia-Sancho J, Giraldez F, Belmonte C (1978) Absence of apparent cell pH variations during hypoxia in the carotid body chemoreceptors in vitro. Neurosci Lett 10:247–249PubMedCrossRefGoogle Scholar
  4. Itturiaga R, Rumsey WL, Lahiri S, Spergel D, Wilson D (1992) Intracellular pH and oxygen chemoreception in the cat carotid body in vitro. J Appl Physiol 72(6):2259–66Google Scholar
  5. Pang L, Eyzaguirre C (1993) Hypoxia affects differently the intracellular pH of clustered and isolated glomus cells of the rat carotid body. Brain Res 623(2):349–355PubMedCrossRefGoogle Scholar
  6. Wilding TJ, Cheng B, Roos A (1992) pH regulation in adult rat carotid body glomus cells. Importance of extracellular pH, sodium, and potassium. J Gen Physiol 100:593–608PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Ryan L. Shapiro
    • 1
  • Barbara L. Barr
    • 1
  • Robert W. Putnam
    • 1
  • Christopher N. Wyatt
    • 2
    Email author
  1. 1.Department of Neuroscience, Cell Biology and PhysiologyWright State UniversityDaytonUSA
  2. 2.Department of Neuroscience, Cell Biology and Physiology, Boonshoft School of MedicineWright State UniversityDaytonUSA

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