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Acute Hypoxia Does Not Influence Intracellular pH in Isolated Rat Carotid Body Type I Cells

  • Ryan L. Shapiro
  • Barbara L. Barr
  • Robert W. Putnam
  • Christopher N. WyattEmail author
Conference paper
  • 3.5k Downloads
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 758)

Abstract

In order to interpret data obtained from isolated Type I cells during hypoxia it is critical to know whether parameters such as intracellular pH (pHi) vary during this challenge. Multiple studies have attempted to address this issue but data are contradictory and methods poorly defined (Wilding et al. 1993; Pang and Eyzaguirre 1993). Wilding et al. used BCECF loaded, isolated Type I cells and demonstrated that ‘hypoxia’ did not cause a change in pHi. However, solutions in these experiments were gassed with 2% oxygen and recording chamber PO2 was not reported. Conversely, Pang and Eyzaguirre used pH-sensitive microelectrodes and demonstrated that single isolated Type I cells did acidify during a hypoxic challenge whereas clusters of Type I cells did not.

Keywords

Carotid body Hypoxia Intracellular pH 

Notes

Acknowledgements

RLS was privately funded by R. M. Johnson MD, T. Perry MD and R. Laughlin MD. CNW was funded by NIH 1RO1HL091836

References

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Ryan L. Shapiro
    • 1
  • Barbara L. Barr
    • 1
  • Robert W. Putnam
    • 1
  • Christopher N. Wyatt
    • 2
    Email author
  1. 1.Department of Neuroscience, Cell Biology and PhysiologyWright State UniversityDaytonUSA
  2. 2.Department of Neuroscience, Cell Biology and Physiology, Boonshoft School of MedicineWright State UniversityDaytonUSA

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