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Hypoxic Redistribution of Iron and Calcium in the Cat Glomus Cells

  • Mieczyslaw PokorskiEmail author
  • Lidia Faff
  • Camillo Di Giulio
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 758)

Abstract

Both iron and calcium are essential for the hypoxia sensing mechanisms in the carotid body. However, trafficking of both ions in chemoreceptor cells in response to hypoxia is unclear. In the present study we seek to determine iron and calcium redistribution patterns in response to hypoxia in the cat chemoreceptor cells. Four cats were used: two each exposed to normoxia (PaO2 = 90 mmHg) and hypoxia (PaO2 = 20 mmHg) for 40 min. Carotid bodies were dissected, 150 nm sections made and processed for the measurements of iron and calcium content in the intracellular organelles of chemoreceptor cells with an energy dispersive X-ray spectroscopy. The results show that iron was distinctly lower in the hypoxic than normoxic chemoreceptor cells’ cytoplasm. Conversely, calcium was increased in hypoxia, particularly in the nuclei and the dense-cored vesicles. These results highlight that regional distribution of iron does not coincide with calcium in glomus cells. Redistribution of both ions in response to hypoxia is congruous with their role in hypoxia-sensing. However, the exact determinants of iron/calcium redistribution patterns in glomus cells remain unsettled.

Keywords

Calcium Carotid body Chemoreceptor cells Hypoxia Iron 

Notes

Acknowledgements

The X-ray spectroscopy was carried out at the Lab for Electron Microscopy, Leiden University in the Netherlands, where L.F. was a fellow at the time of the study. The authors are thankful to the Dutch collaborators for help in the measurements.

Conflicts of interest The authors declare no conflicts of interest in relation to this article.

References

  1. Baby SM, Roy A, Mokashi AM, Lahiri S (2003) Effects of hypoxia and intracellular iron chelation on hypoxia-inducible factor-1α and -1β in the rat carotid body and glomus cells. Histochem Cell Biol 120:343–352PubMedCrossRefGoogle Scholar
  2. Buerk DG, Osanai S, Chugh DK, Mokashi A, Lahiri S (1997) Calcium-dependent O2 sensitivity of cat carotid body. Adv Exp Med Biol 411:1–5PubMedCrossRefGoogle Scholar
  3. Conde SV, Caceres AI, Vicario I, Rocher A, Obeso A, Gonzalez C (2006) An overview on the homeostasis of Ca2+ in glomus cells of the rabbit and rat carotid bodies. Adv Exp Med Biol 580:215–222PubMedCrossRefGoogle Scholar
  4. Daudu PA, Roy A, Rozanov C, Mokashi A, Lahiri S (2002) Extra- and intracellular free iron and the carotid body responses. Respir Physiol Neurobiol 130:21–31PubMedCrossRefGoogle Scholar
  5. Faff L, van der Meulen H, Koerten HK, Walski M, Pokorski M (2001) Calcium handling by the cat carotid body – a pyroantimonate study. Acta Hisotochem 103:305–313CrossRefGoogle Scholar
  6. Fandrey J, Gassmann M (2009) Oxygen sensing and the activation of the hypoxia inducible factor 1 (HIF-1). Adv Exp Med Biol 648:197–206PubMedCrossRefGoogle Scholar
  7. Kell DB (2009) Iron behaving badly: inappropriate iron chelation as a major contributor to the aetiology of vascular and other progressive inflammatory and degenerative diseases. BMC Med Genomics 2:2. doi: 10.1186/1755-8794-2-2 PubMedCrossRefGoogle Scholar
  8. Nemeth E, Tuttle MS, Powelson J, Vaughn MB, Donovan A, Ward DM, Ganz T, Kaplan J (2004) Hepcidin regulates cellular iron efflux by binding to ferroportin and inducing its internalization. Science 306:2090–2093PubMedCrossRefGoogle Scholar
  9. Nguyen MVC, Pouvreau S, El Hajjaji FZ, Denavit-Saubie M, Pequignot JM (2007) Desferrioxamine enhances hypoxic ventilatory response and induces tyrosine hydroxylase gene expression in the rat brainstem in vivo. J Neurosci Res 85:1119–1125PubMedCrossRefGoogle Scholar
  10. Nicolas G, Chauvet C, Viatte L, Danan JL, Bigard X, Devaux I, Beaumont C, Kahn A, Vaulont S (2002) The gene encoding the iron regulatory peptide hepcidin is regulated by anemia, hypoxia, and inflammation. J Clin Invest 110:1037–1044PubMedGoogle Scholar
  11. Pokorski M, Sakagami H, Kondo H (2000) Classical protein kinase C and its hypoxic stimulus-induced translocation in the cat and rat carotid body. Eur Respir J 16:459–463PubMedCrossRefGoogle Scholar
  12. Pokorski M, Antosiewicz J, Di Giulio C, Lahiri S (2009) Iron chelation and the ventilatory response to hypoxia. Adv Exp Med Biol 648:215–221PubMedCrossRefGoogle Scholar
  13. Roy A, Li J, Baby SM, Mokashi A, Burek DG, Lahiri S (2004a) Effects of iron-chelators on ion-channels and HIF-1α in the carotid body. Respir Physiol Neurobiol 141:115–123PubMedCrossRefGoogle Scholar
  14. Roy A, Volgin DV, Baby SM, Mokashi A, Kubin L, Lahiri S (2004b) Activation of HIF-1α mRNA by hypoxia and iron chelator in isolated rat carotid body. Neurosci Lett 363:229–232PubMedCrossRefGoogle Scholar
  15. Strosznajder RP, Pokorski M (2000) Regulation of phospholipase C activity by calcium ions and guanine nucleotide in the normoxic cat carotid body. Neurochem Res 25:739–743PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Mieczyslaw Pokorski
    • 1
    Email author
  • Lidia Faff
    • 1
  • Camillo Di Giulio
    • 2
  1. 1.Department of Respiratory Research, Medical Research CenterPolish Academy of SciencesWarsawPoland
  2. 2.Department of Neuroscience and ImagingUniversity of Chieti-Pescara, ‘G. d’Annunzio’ChietiItaly

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