Abstract
This chapter describes methodology to exploit the murine hindbrain as a powerful and versatile model system with three main advantages for studying developmental angiogenesis. Firstly, the hindbrain model permits the accurate assessment of the intricate behaviour of endothelial cells within a natural multicellular microenvironment. Secondly, due to the early embryonic vascularisation of the hindbrain, it is particularly useful to study genetic mutations that result in embryonic lethality, reducing the need to breed conditional knockout mice, as for postnatal models. Thirdly, the unique architecture of the vascular network in this tissue allows high resolution imaging of angiogenic molecules and vascular structures that are readily quantifiable.
An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-94-007-4581-0_26
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- HRP:
-
Horseradish peroxidase
- IB4:
-
Isolectin B4
- PECAM:
-
Platelet endothelial cell adhesion molecule
- NGS:
-
Normal goat serum
- NRS:
-
Normal rabbit serum
- PBS:
-
Phosphate buffered saline
- PBT:
-
PBS + 0.1% TritonX100
- PFA:
-
Paraformaldehyde
- SVP:
-
Subventricular vascular plexus
- VEGF:
-
Vascular endothelial growth factor
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Acknowledgements
We thank Dr Alessandro Fantin for Fig. 1 and critical reading of the manuscript. We are grateful to L. Denti, K. Davidson and the staff of the Biological Resources Unit at the UCL Institute of Ophthalmology for help with mouse husbandry. We gratefully acknowledge the Imaging Facility of the UCL Institute of Ophthalmology for maintenance of the confocal microscopes. C.M. was funded by an MRC doctoral training account (ref. G0601093).
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© 2012 Springer Science+Business Media Dordrecht
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Maden, C., Ruhrberg, C. (2012). The Murine Hindbrain as a Model to Study the Molecular and Cellular Mechanisms of Angiogenesis in Intact Tissues. In: Zudaire, E., Cuttitta, F. (eds) The Textbook of Angiogenesis and Lymphangiogenesis: Methods and Applications. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4581-0_13
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DOI: https://doi.org/10.1007/978-94-007-4581-0_13
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