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The Structure and Function of Replication Protein A in DNA Replication

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The Eukaryotic Replisome: a Guide to Protein Structure and Function

Part of the book series: Subcellular Biochemistry ((SCBI,volume 62))

Abstract

In all organisms from bacteria and archaea to eukarya, single-stranded DNA binding proteins play an essential role in most, if not all, nuclear metabolism involving single-stranded DNA (ssDNA). Replication protein A (RPA), the major eukaryotic ssDNA binding protein, has two important roles in DNA metabolism: (1) in binding ssDNA to protect it and to keep it unfolded, and (2) in coordinating the assembly and disassembly of numerous proteins and protein complexes during processes such as DNA replication. Since its discovery as a vital player in the process of replication, RPAs roles in recombination and DNA repair quickly became evident. This chapter summarizes the current understanding of RPA’s roles in replication by reviewing the available structural data, DNA-binding properties, interactions with various replication proteins, and interactions with DNA repair proteins when DNA replication is stalled.

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Acknowledgements

This work was supported by the American Cancer Society [RSG-02-162-01-GMC], NCI Eppley Cancer Center Support Grant [P30CA036727] and the Nebraska Department of Health and Human Services grants [2011-05 & 2012-04]. Aishwarya Prakash was supported by a University of Nebraska Medical Center graduate fellowship and Presidential graduate fellowship and would also like to thank Dr. Sylvie Doubliè for her support [NIH/NCI P01CA098993].

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Authors and Affiliations

  1. Department of Microbiology and Molecular Genetics, The Markey Center for Molecular Genetics, University of Vermont, Given Medical Building, 89 Beaumont Avenue, Burlington, VT, 05405, USA

    Aishwarya Prakash

  2. The Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 987696 Nebraska Medical Center, Omaha, NE, 68198-7696, USA

    Gloria E. O. Borgstahl

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  1. Aishwarya Prakash
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  2. Gloria E. O. Borgstahl
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Correspondence to Gloria E. O. Borgstahl .

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  1. , School of Biology, University of St Andrews, Biomedical Sciences Research Complex, North Haugh, St Andrews, KY16 9ST, Fife, United Kingdom

    Stuart MacNeill

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Prakash, A., Borgstahl, G.E.O. (2012). The Structure and Function of Replication Protein A in DNA Replication. In: MacNeill, S. (eds) The Eukaryotic Replisome: a Guide to Protein Structure and Function. Subcellular Biochemistry, vol 62. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4572-8_10

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