Abstract
DArT technology was applied to a tetraploid (2n = 4x = 28) L. multiflorum´F. glaucescens hybrid lineage over ten generations. By selecting 151 Lolium- and 210 Festuca-specific DArT markers among the 3,884 ones developed by Kopecký et al. (Development and mapping of DArT markers within the Festuca–Lolium complex, BMC Genomics 10:473, 2009), it is shown that DArT polymorphism is well consistent with the history of the plant material and the events in relation with interspecific hybridisation: amphiploidisation, introgression into 4x- and 2x-L. multiflorum, reduction of effective size, chromosome mapping as well as parent-specific response to summer water deficit. In this respect, frequency of Festuca markers within a 4x-BC1 population was found to have increased on average by 2.4 % among plants having survived in sward after summer while frequency decreased by 10.2 % after three generations of seed multiplication from the initial polycross of BC1 parents.
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The authors thank Denise Cadier, Françoise Durand and Claudine Largeaud for help and careful management of all living plants and DNA materials required for this study.
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Ghesquière, M., Durand, JL., Bourgoin, T., Huttner, E., Kilian, A. (2013). Using DArT Markers in Festuca × Lolium Breeding. In: Barth, S., Milbourne, D. (eds) Breeding strategies for sustainable forage and turf grass improvement. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4555-1_15
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DOI: https://doi.org/10.1007/978-94-007-4555-1_15
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