Abstract
Adiponectin plays a protective role against atherosclerosis. Genetic investigation has revealed that G276T adiponectin gene polymorphism is related to adiponectin concentration and metabolic disturbances. The aim of the present study was to investigate the association of adiponectin gene G276T polymorphism with indices of atherosclerosis in obese children. We examined 159 children (125 obese and 34 non-obese). G276T of adiponectin gene polymorphism was identified using a PCR-RFLP method. The intima media thickness (IMT) was evaluated in 82 patients. In all children, the anthropometric indices, fasting plasma total cholesterol (TC), HDL and LDL cholesterol, triglycerydes (TG), C-reactive protein (CRP), and adiponectin were measured. Oral glucose tolerance test (OGTT) also was performed. We found that the obese patients presented with higher values of atherogenic indicators than the non-obese patients. The indicators positively correlated with CRP and lipid concentrations. Ninety one percent of obese children presented with elevated IMT which correlated with CRP. The children with GG genotype (GG + GT allele) had lower values of BMI, TC, and TG but higher adiponectin concentrations. The mean level of adiponectin was statistically decreased in the compared with the homozygous TT children. The other anthropometric and atherogenic indicators did not differ between these two sets of obese children. We conclude that adiponectin concentrations were decreased in children with polymorphism G276T in adiponectin gene. The study, however, failed to show significant associations between carotid IMT, lipid markers, blood pressure, or HOMA-IR in obese children.
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References
Arnaiz, P., Acevedo, M., Barja, S., Aglony, M., Guzmán, B., Cassis, B., Carvajal, J., Moreno, M., Navarrete, C., & BerrÃos, X. (2010). Adiponectin levels, cardiometabolic risk factors and markers of subclinical atherosclerosis in children. International Journal of Cardiology, 138(2), 138–144.
Bouatia-Naji, N., Meyre, D., Lobbens, S., Séron, K., Fumeron, F., Balkau, B., Heude, B., Jouret, B., Scherer, P. E., Dina, C., Weill, J., & Froguel, P. (2005). ACDC/adiponectin polymorphisms are associated with severe childhood and adult obesity. Diabetes, 55(2), 545–550.
Cieslak, J., Skorczyk, A., Stachowiak, M., Szydłowski, M., Grzes, M., Paczynska, P., Skowronska, B., Majewska, K., Stankiewicz, W., Fichna, P., & Switoński, M. (2011). Polymorphisms in 5′-flanking regions of genes encoding adiponectin, leptin, and resistin are not associated with obesity of polish children and adolescents. Molecular Biology Reports, 38(3), 1793–1798.
Cordell, H. J. (2009). Genome-wide association studies: Detecting gene-gene interactions that underlie human diseases. Nature Reviews Genetics, 10, 392–404.
Diez, J. J., & Iglesias, P. (2003). The role of the novel adipocyte-derived hormone adiponectin in human disease. European Journal of Endocrinology, 148, 293–300.
Fan, Y. M., Raitakari, O. T., Kähönen, M., Hutri-Kähönen, N., Juonala, M., Marniemi, J., Viikari, J., & Lehtimäki, T. (2009). Hepatic lipase promoter C-480T polymorphism is associated with serum lipids levels, but not subclinical atherosclerosis: The Cardiovascular Risk in Young Finns Study. Clinical Genetics, 76, 46–53.
Gibson, F., & Froguel, P. (2004). Genetics of the APM1 locus and its contribution to type 2 diabetes susceptibility in French Caucasians. Diabetes, 53, 2977–2983.
Hara, K., Boutin, P., Mori, Y., Tobe, K., Dina, C., Yasuda, K., Yamauchi, T., Otabe, S., Okada, T., Eto, K., Kadowaki, H., Hagura, R., Akanuma, Y., Yazaki, Y., Nagai, R., Taniyama, M., Matsubara, K., Yoda, M., Nakano, Y., Tomita, M., Kimura, S., Ito, C., Froguel, P., & Kadowaki, T. (2002). Genetic variation in the gene encoding adiponectin is associated with an increased risk of type 2 diabetes in the Japanese population. Diabetes, 51(2), 536–540.
Heid, I. M., Wagner, S. A., Gohlke, H., Iglseder, B., Mueller, J. C., Cip, P., Ladurner, G., Reiter, R., Stadlmayr, A., Mackevics, V., Illig, T., Kronenberg, F., & Paulweber, B. (2006). Genetic architecture of the APM1 gene and its influence on adiponectin plasma levels and parameters of the metabolic syndrome in 1,727 healthy Caucasians. Diabetes, 55, 375–384.
Hosking, L., Lumsden, S., Lewis, K., Yeo, A., McCarthy, L., Bansal, A., Riley, J., Purvis, I., & Xu, C. (2004). Detection of genotyping errors by Hardy-Weinberg equilibrium testing. European Journal of Human Genetics, 12, 395–399.
Humphries, S. E., Cooper, J. A., Talmud, P. J., & Miller, G. J. (2007). Candidate gene genotypes, along with conventional risk factor assessment, improve estimation of coronary heart disease risk in healthy UK men. Clinical Chemistry, 53, 8–16.
Jansson, P. A., Pellme, F., Hammarstedt, A., Sandqvist, M., Brekke, H., Caidahl, K., Forsberg, M., Volkmann, R., Carvalho, E., Funahashi, T., Matsuzawa, Y., Wiklund, O., Yang, X., Taskinen, M. R., & Smith, U. (2003). A novel cellular marker of insulin resistance and early atherosclerosis in humans is related to impaired fat cell differentiation and low adiponectin. The FASEB Journal, 17, 1434–1440.
Kazumi, T., Kawaguchi, A., Sakai, K., Hirano, T., & Yoshino, G. (2002). Young men with high-normal blood pressure have lower serum adiponectin smaller LDL size, and higher elevated heart rate than that with optimal blood pressure. Diabetes Care, 25, 971–976.
Kondo, H., Shimomura, I., Matsukawa, Y., Kumada, M., Takahashi, M., Matsuda, M., Ouchi, N., Kihara, S., Kawamoto, T., Sumitsuji, S., Funahashi, T., & Matsuzawa, Y. (2002). Association of adiponectin mutation with type 2 diabetes. A candidate gene for the insulin resistance syndrome. Diabetes, 51, 2325–2328.
Lorenz, M. W., Markus, H. S., Bots, M. L., Rosvall, M., & Sitzer, M. (2007). Prediction of clinical cardiovascular events with carotid intima-media thickness: A systematic review and meta-analysis. Circulation, 115, 459–467.
Matsuda, M., Kawasami, F., Hamada, K., Kanda, Y., Saito, M., Eto, M., Matsuki, M., & Kaku, K. (2004). Impact of adiposity and plasma adipocytokines on diabetic angiopathies in Japanese Type 2 diabetic subjects. Diabetic Medicine, 21, 881–888.
Mori, Y., Otabe, S., Dina, C., Yasuda, K., Populaire, C., Lecoeur, C., Vatin, V., Durand, E., Hara, K., Okada, T., Tobe, K., Boutin, P., Kadowaki, T., & Froguel, P. (2002). Genome-wide search for type 2 diabetes in Japanese affected sib-pairs confirms susceptibility genes on 3q, 15q, and 20q and identifies two new candidate Loci on 7p and 11p. Diabetes, 51(4), 1247–1255.
O’Leary, D. H., Polak, J. F., Kronmal, R. A., Magnolio, T. A., & Burke, G. L. (1999). Carotid-artery intima and media thickness as a risk factor for myocardial infarction and stroke in older adults. Cardiovascular Health Study Collaborative Research Group. The New England Journal of Medicine, 340, 14–22.
Ogawa, Y., Kikuchi, T., Nagasaki, K., Hiura, M., Tanaka, Y., & Uchiyama, M. (2005). Usefulness of serum adiponectin level as a diagnostic marker of metabolic syndrome in obese Japanese children. Hypertension Research, 28, 51–57.
Ouchi, N., Kihara, S., Arita, Y., Maeda, K., Kuriyama, H., Okamoto, Y., Hotta, K., Nishida, M., Takahashi, M., Nakamura, T., Yamashita, S., Funahashi, T., & Matsuzawa, Y. (1999). Novel modulator for endothelial adhesion molecules: Adipocyte-derived plasma protein adiponectin. Circulation, 100, 2473–2476.
Ouchi, N., Kihara, S., Arita, Y., Nishida, M., Matsuyama, A., Okamoto, Y., Ishigami, M., Kuriyama, H., Kishida, K., Nishizawa, H., Hotta, K., Muraguchi, M., Ohmoto, Y., Yamashita, S., Funahashi, T., & Matsuzawa, Y. (2001). Adipocyte-derived plasma protein, adiponectin suppresses lipid accumulation and class A scavenger receptor expression in human monocyte-derived macrophages. Circulation, 103, 1057–1063.
Panagopoulou, P., Galli-Tsinopoulou, A., Fleva, A., Pavlitou-Tsiontsi, E., Vavatsi-Christaki, N., & Nousia-Arvanitakis, S. (2008). Adiponectin and insulin resistance in childhood obesity. Journal of Pediatric Gastroenterology and Nutrition, 47, 356–362.
Pollin, T. I., Tanner, K., O’connell, J. R., Ott, S. H., Damcott, C. M., Shuldiner, A. R., McLenithan, J. C., & Mitchell, B. D. (2005). Linkage of plasma adiponectin levels to 3q27 explained by association with variation in the APM1 gene. Diabetes, 54, 268–274.
Qi, L., Li, T., Rimm, E., Zhang, C., Rifai, N., Hunter, D., Doria, A., & Hu, F. B. (2005). The 276 polymorphism of the APM1 gene, plasma adiponectin concentration, a cardiovascular risk in diabetic men. Diabetes, 54, 1607–1610.
Samani, N. J., Raitakari, O. T., Sipila, K., Tobin, M. D., Schunkert, H., Juonala, M., Braund, P. S., Erdmann, J., Viikari, J., Moilanen, L., Taittonen, L., Jula, A., Jokinen, E., Laitinen, T., Hutri-Kähönen, N., Nieminen, M. S., Kesäniemi, Y. A., Hall, A. S., Hulkkonen, J., Kähönen, M., & Lehtimäki, T. (2008). Coronary artery disease-associated locus on chromosome 9p21 and early markers of atherosclerosis. Arteriosclerosis, Thrombosis, and Vascular Biology, 28, 1679–1683.
Slaughter, M. H., Lohman, T. G., Boileau, R. A., Horswill, C. A., Stillman, R. J., Van Loan, M. D., & Bemben, D. A. (1988). Skinfold equations for estimation of body fatness in children and youth. Human Biology, 60, 709–723.
Trujilio, M. E., & Scerer, P. E. (2005). Adiponectin-journey from an adipocyte secretory protein to biomarker of the metabolic syndrome. Journal of Internal Medicine, 257, 167–175.
Vozarova de Courten, B., Hanson, R. L., Funahashi, T., Lindsay, R. S., Matsuzawa, Y., Tanaka, S., Thameem, F., Gruber, J. D., Froguel, P., & Wolford, J. K. (2005). Common polymorphisms in the adiponectin gene ACDC are not associated with diabetes in Pima Indians. Diabetes, 54, 284–289.
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Pyrzak, B. et al. (2013). Association of Adiponectin Gene G276T Polymorphism with Atherogenic Indicators in Obese Children. In: Pokorski, M. (eds) Respiratory Regulation - The Molecular Approach. Advances in Experimental Medicine and Biology, vol 756. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4549-0_31
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