Abstract
Tobacco cDNA clones representing genes differentially regulated by the short-term sulfur deficit were identified with the suppression subtractive hybridization (SSH) method. Many of them encoded proteins with established function, however for some of them not only their role in sulfur deficit response remained elusive but assigning them to any cellular processes was impossible. The down-regulated D6 clone was representing one of such genes. The first steps of analysis included cloning of the full-length mRNA sequence and the sequence of its promoter region. Subsequent work, including monitoring of D6 expression in various growth conditions, its intracellular localization and database screening for homologues lead to the hypothesis that D6 may take part in differentiation and developing processes. However, its precise role and rationale for down-regulation during sulfur deficit remain unclear.
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Bristow J, Carey W, Egging D, Schalkwijk J (2005) Tenascin-X, collagen, elastin, and the Ehlers–Danlos syndrome. Am J Med Genet C Semin Med Genet 139C:24–30
Demidova-Rice TN, Geevarghese A, Herman IM (2011) Bioactive peptides derived from vascular endothelial cell extracellular matrices promote microvascular morphogenesis and wound healing in vitro. Wound Repair Regen 19:59–70
Egging D, van Vlijmen-Willems I, van Tongeren T, van Tongeren T, Schalkwijk J, Peeters A (2007) Wound healing in tenascin-X deficient mice suggests that tenascin-X is involved in matrix maturation rather than matrix deposition. Connect Tissue Res 48:93–98
Fujita T, Kouchi H, Ichikawa T, Syono K (1994) Cloning of cDNAs for genes that are specifically or preferentially expressed during the development of tobacco genetic tumors. Plant J 5:645–654
Lewandowska M, Sirko A (2009) Identification of additional genes regulated by sulfur shortage in tobacco. In: Sirko A, De Kok LJ, Haneklaus S, Hawkesford MJ, Rennenberg H, Saito K, Schnug E, Stulen I (eds) Sulfur metabolism in plants. Regulatory aspects, significance of sulfur in the food chain, agriculture and the environment. Backhuys Publishers, Leiden, Margraf Publishers GmbH, Weikersheim, pp 73–77
Lewandowska M, Wawrzyńska A, Kamińska J, Liszewska F, Sirko A (2005) Identification of novel proteins of Nicotiana tabacum regulated by short term sulfur starvation. In: Saito K, De Kok LJ, Stulen I, Hawkesford MJ, Schnug E, Sirko A, Rennenberg H (eds) Sulfur transport and assimilation in plants in the postgenomic era. Backhuys Publishers, Leiden, pp 153–156
Lewandowska M, Wawrzyńska A, Moniuszko G, Łukomska J, Zientara K, Piecho M, Hodurek P, Zhukov I, Liszewska F, Nikiforova V, Sirko A (2010) A contribution to identification of novel regulators of plant response to sulfur deficiency: characteristics of a tobacco gene UP9C, its protein product and the effects of UP9C silencing. Mol Plant 3:347–360
Maruyama-Nakashita A, Nakamura Y, Tohge T, Saito K, Takahashi H (2006) Arabidopsis SLIM1 is a central transcriptional regulator of plant sulfur response and metabolism. Plant Cell 18:3235–3251
Meiniel O, Meiniel R, Lalloue F, Didier R, Jauberteau M-O, Meiniel A, Petit D (2008) The lengthening of a giant protein: when, how, and why? J Mol Evol 66:1–10
Nacken WK, Huijser P, Beltran JP, Saedler H, Sommer H (1991) Molecular characterization of two stamen-specific genes, tap1 and fil1, that are expressed in the wild type, but not in the deficiens mutant of Antirrhinum majus. Mol Gen Genet 229:129–136
Wawrzyńska A, Lewandowska M, Hawkesford MJ, Sirko A (2005) Using a suppression subtractive library-based approach to identify tobacco genes regulated in response to short-term sulphur deficit. J Exp Bot 56:1575–1590
Wawrzyńska A, Lewandowska M, Sirko A (2010) Nicotiana tabacum EIL2 directly regulates expression of at least one tobacco gene induced by sulphur starvation. J Exp Bot 61:889–900
Zientara-Rytter K, Łukomska J, Moniuszko G, Gwozdecki R, Surowiecki P, Lewandowska M, Liszewska F, Wawrzyńska A, Sirko A (2011) Identification and functional analysis of Joka2, a tobacco member of the family of selective autophagy cargo receptors. Autophagy 7:1145–1158
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The work was supported by the Ministry of Science and Higher Education (N N302 119435). ML acknowledges support by German Academic Exchange Service (DAAD).
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Lewandowska, M., Zientara-Rytter, K., Sirko, A. (2012). Preliminary Characteristics of a Tobacco Gene Down-Regulated by Sulfur Deprivation and Encoding a Cys-Rich Protein. In: De Kok, L., et al. Sulfur Metabolism in Plants. Proceedings of the International Plant Sulfur Workshop, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4450-9_10
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DOI: https://doi.org/10.1007/978-94-007-4450-9_10
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