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Characterization of Bacterial Complex I (NDH-1) by a Genetic Engineering Approach

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A Structural Perspective on Respiratory Complex I

Abstract

Genetic engineering has been widely adopted in many different fields of research and study on complex I is no exception. Because of current limitations in the technology when studying eukaryotes, most work has been performed using bacterial complex I (NDH-1). The bacterial system has been proven to be an excellent model in which to investigate the structure and mechanism of complex I. Sequence information, from the early days, and the availability of 3D structures, in more recent years, of NDH-1 have significantly boosted the importance of the gene engineering approach. Mutational approach has helped in identifying a number of amino acid residues that are essential not only for structural integrity but also for cofactor ligation, substrate binding and electron transfer. More recently, this approach determined residues essential for proton translocation coupled to the electron transfer from NADH to quinone. Understanding the roles of these residues, together with structural information, should lead to the elucidation of the reaction mechanism of NDH-1/complex I. This chapter is concerned with that knowledge of NDH-1 which has been advanced by a genetic engineering approach and possible applications that may extend the research to mitochondrial complex I.

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Authors and Affiliations

  1. The Scripps Research Institute, MEM-256, 10550 North Torrey Pines Rd., La Jolla, CA, 92037, USA

    Takao Yagi, Jesus Torres-Bacete, Prem Kumar Sinha, Norma Castro-Guerrero & Akemi Matsuno-Yagi

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  1. Takao Yagi
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  2. Jesus Torres-Bacete
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  3. Prem Kumar Sinha
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  4. Norma Castro-Guerrero
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  5. Akemi Matsuno-Yagi
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Correspondence to Takao Yagi .

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  1. , Mitochondrial Biology Unit, Medical Research Council, Hills road, Cambridge, CB2 0XY, Cambridgeshire, United Kingdom

    Leonid Sazanov

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Yagi, T., Torres-Bacete, J., Sinha, P.K., Castro-Guerrero, N., Matsuno-Yagi, A. (2012). Characterization of Bacterial Complex I (NDH-1) by a Genetic Engineering Approach. In: Sazanov, L. (eds) A Structural Perspective on Respiratory Complex I. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4138-6_8

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