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Agrobacterium tumefaciens and its Use in Plant Biotechnology

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Crop Production for Agricultural Improvement

Abstract

For years, isolation of foreign genes from one plant and transferring them to another and then observing effects of new genes in transferred plants has only been a dream for a plant biologist. Today, many commercially important species are routinely transformed by different biotechnological methods. Methods available for plant transformation are arranged in three main groups: using biological vectors (virus- or bacteria-mediated transformation), direct DNA transfer techniques (chemical-, electrical-, or laser-induced permeability of protoplasts or cells) and non-biological vector systems (microprojectiles, microinjection or liposome fusion). Today in many countries a number of transgenic important crops such as soybean, maize, cotton, canola, sugarbeet, sugarcane and alfalfa are available and the mostly preferred method is Agrobacterium-mediated transformation. In this chapter, some information about this important bacterium and mechanisms of Agrobacterium-mediated gene transfer are presented.

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Acknowledgement

I would like to express my special thanks to Professor Sebahattin Özcan (Ankara University, Agronomy Department), Dr. İlhan Doğan (Izmir Institute of Technology, Department of Molecular Biology and Genetics), Dr. Ülkühan Yaşar (Bartin University, Department of Environmental Engineering) and Dr. Göksel Demir (Bahçeşehir University, Department of Environmental Engineering) for a critical reading of this chapter and for their valuable suggestions. My thanks go to İlke Ertem (Biologist) for his assistance in drawing figures, Melike Isci (Marmara University) for rearranging the references. I am also thankful to Dr. Ayse Zeynep Isik for linguistic corrections and Bahçeşehir University, for their technical support.

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  1. Science & Arts Faculty, Department of Biology, Marmara University, Göztepe-Istanbul, Turkey

    İbrahim İlker Özyiğit

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  1. İbrahim İlker Özyiğit
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Correspondence to İbrahim İlker Özyiğit .

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  1. Faculty of Sciences, University of Agriculture, Faisalabad, Pakistan

    Muhammad Ashraf

  2. Center for Environmental Studies, Ege University, Bornova, Izmir, 35100, Turkey

    Münir Öztürk

  3. , Department of Botany, University of Agriculture Faisalabad, Jail Road, Faisalabad, 38040, Pakistan

    Muhammad Sajid Aqeel Ahmad

  4. , Department of Biology, Erciyes University, Rektörlüğü, Melikgazi, 38039, Kayseri, Turkey

    Ahmet Aksoy

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Özyiğit, İ.İ. (2012). Agrobacterium tumefaciens and its Use in Plant Biotechnology. In: Ashraf, M., Öztürk, M., Ahmad, M., Aksoy, A. (eds) Crop Production for Agricultural Improvement. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4116-4_12

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