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Ion Exchange Technology II pp 391–411Cite as

Cation-Exchanged Silica Gel–Based Thin-Layer Chromatography of Organic and Inorganic Compounds

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Abstract

The main goal of this chapter is to encapsulate the data published during last 27 years (1984–2010) on cation-exchanged silica gel–based thin-layer chromatography (TLC) of organic and inorganic species. It includes the types of impregnating cations/salts, mobile phase, and technique involved in the separation, identification, and determination of organic/inorganic substances present either singly or as components of closely related mixtures in a variety of matrices. The use of cation-exchanged silica gel layers in TLC/HPTLC analyses of organic and inorganic species is described by citing several examples. It has been noticed that little attention has been paid on the use of cation-exchanged silica gel as layer material in the analysis of inorganic substances as compared to organic compounds. The alternative features of using cation-exchanged silica gel layers include the better differential migration, increase separation efficiency, and improved resolution of analytes. According to the literature survey, the interest of chromatographers in using the cations/salts for impregnation of silica gel layers has been in the following order: \( \text{Ag > Cu > Ni > Mn > Fe}\approx \text{Zn > Co}\text{.} \)

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References

  1. Kirchner JG, Miller JM, Keller GJ (1951) Separation and identification of some terpenes by a new chromatographic technique. Anal Chem 23:420–425

    Article  CAS  Google Scholar 

  2. Stahl E (1956) Thin-layer chromatography. Pharmazie 11:633–637

    CAS  Google Scholar 

  3. Barrett CB, Dallas MSJ, Padley FB (1962) Chem Ind (London) 1050–1052

    Google Scholar 

  4. Stuhlfauth T, Fock H, Huber H, Klug K (1985) The distribution of fatty acids including petroselinic and tariric acids in the fruit and seed oils of the Pittosporaceae, Araliaceae, Umbelliferae, Simarubaceae and Rutaceae. Biochem Syst Ecol 13:447–453

    Article  CAS  Google Scholar 

  5. Sventashev VI, Zhukova NV (1985) Analysis of labelled fatty acid methyl esters by argentation and reversed-phase two-dimensional thin layer chromatography. J Chromatogr 330:396–399

    Article  Google Scholar 

  6. Xu S, Norton RA, Crumley FG, Nes WD (1988) Comparison of the chromatographic properties of sterols, select additional steroids and triterpenoids: gravity-flow column liquid chromatography, thin layer chromatography, gas liquid chromatography and high performance liquid chromatography. J Chromatogr 452:377–398

    Article  CAS  Google Scholar 

  7. Michalec C (1990) Argentation TLC and HPTLC of cholesterol and related stanols and stanones. J Planar Chromatogr 3:273–275

    CAS  Google Scholar 

  8. Mares P, Rezanka T, Novak M (1991) Analysis of human blood plasma triacylglycerols using capillary gas chromatography. Silver ion thin layer chromatographic fractionation and desorption chemical ionization mass spectrometry. J Chromatogr 568:1–10

    Article  CAS  Google Scholar 

  9. Chobanov D, Tarandjiiska R, Nikolova-Damyanove B (1992) Quantitation of isomeric unsaturated fatty acids by argentation TLC. J Planar Chromatogr 5:157–163

    CAS  Google Scholar 

  10. Masterson Ch, Fried B, Sherma J (1992) Comparison of mobile phase and detection reagents for the separation of triacylglycerols by silica gel, argentation, and reversed phase thin layer chromatography. J Liq Chromatogr Rel Technol 15:2967–2980

    Article  CAS  Google Scholar 

  11. Wilson R, Sargent JR (1992) High resolution separation of polyunsaturated fatty acids by argentation thin layer chromatography. J Chromatogr 623:403–407

    Article  CAS  Google Scholar 

  12. Sempore BG, Bezard JA (1994) Separation of monoacylglycerol enantiomers as urethane derivatives by chiral-phase high performance liquid chromatography. J Liq Chromatogr Rel Technol 17:1679–1694

    Article  CAS  Google Scholar 

  13. Martinez-lorenzo MJ, Marzo I, Naval J, Pineiro A (1994) Self-staining of polyunsaturated fatty acids in argentation thin layer chromatography. Anal Biochem 220:210–212

    Article  CAS  Google Scholar 

  14. Wardas W, Pyka A (1994) Visualizing agents for cholesterol in TLC. J Planar Chromatogr 7:440–443

    CAS  Google Scholar 

  15. Nguyen H, Tarndjiiska R (1995) Lipid classes, fatty acids and triglycerides in papaya seed oil. Fat Sci Technol 97:20–23

    CAS  Google Scholar 

  16. Molkentin J, Precht D (1995) Optimized analysis of trans-octadecenoic acids in edible fats. Chromatrographia 41:267–272

    Article  CAS  Google Scholar 

  17. Dobson G, Christie WW, Nikolova-Damyanova B (1995) Silver ion chromatography of lipids and fatty acids. J Chromatogr B 671:197–222

    Article  CAS  Google Scholar 

  18. Wall PE (1997) Argentation HPTLC as an effective separation technique for the cis/trans isomers of capsaicin. J Planar Chromatogr 10:4–9

    CAS  Google Scholar 

  19. Wardas W, Pyka A (1998) Visualizing agents for cholesterol derivatives in TLC. J Planar Chromatogr 11:70–73

    CAS  Google Scholar 

  20. Wilson R, Sargent JR (2000) Chain separation of monounsaturated fatty acid methyl esters by argentation TLC. J Chromatogr A 905:251–257

    Google Scholar 

  21. Nikolova-Damyanova B, Momchilova S (2001) Silver ion thin layer chromatography of fatty acids. A survey. J Liq Chromatogr Rel Technol 24:1447–1466

    Article  CAS  Google Scholar 

  22. Chrisrie WW (1982) Lipid analysis. Pergamon, Oxford

    Google Scholar 

  23. Kohli JC, Badaisha KK (1985) Improved procedure for the thin layer chromatography of terpenoids on silver ion-silica gel layer. J Chromatogr 320:455–456

    Article  CAS  Google Scholar 

  24. Kennerly DA (1986) Improved analysis of species of phospholipids using argentation thin layer chromatography. J Chromatogr 363:462–467

    Article  CAS  Google Scholar 

  25. Kennerly DA (1987) Molecular species analysis lysophospholipids using high performance liquid chromatography and argentation thin layer chromatography. J Chromatogr 409:291–297

    Article  CAS  Google Scholar 

  26. Kennerly DA (1988) Two dimensional thin layer chromatographic separation of phospholipid molecular species using plates with both reversed-phase and argentation zones. J Chromatogr 454:425–431

    Article  CAS  Google Scholar 

  27. Schick PK, Levy C (1990) Reversed phase and argentation HPTLC for the separation of fatty acids and fatty aldehyde. J Planar Chromatogr 3:269–271

    CAS  Google Scholar 

  28. Nikolova-Damyanove B, Amidzhin B (1991) Densitometric quantitation of triglycerides. J Planar Chromatogr 4:397–401

    Google Scholar 

  29. Bortolomeazzi R, Pizzale L, Lercker G (1992) Chromatographic determination of the position and configuration of isomers of methyl oleate hydroperoxides. J Chromatogr 626:109–116

    Article  CAS  Google Scholar 

  30. Nikolova-Damyanova B, Christie WW, Herslolf B (1994) Improved separation of some positional isomers of monounsaturated fatty acids, as their phenacyl derivatives, by silver ion thin-layer chromatography. J Planar Chromatogr 7:382–385

    CAS  Google Scholar 

  31. Li T-S, Li Ji-Tai, Li H-Z (1995) Modified and convenient preparation of silica impregnated with silver nitrate and its application to the separation of steroids and triterpenes. J Chromatogr A 715:372–375

    Article  CAS  Google Scholar 

  32. Tauaka T, Shibata K, Hino H, Murashita T, Kayama M, Satouche K (1997) Purification and gas chromatographic-mass spectrometric characterization of non-methylene interrupted fatty acid incorporated in rat liver. J Chromatogr B 700:1–8

    Article  Google Scholar 

  33. Fraga MJ, Fontecha J, Lozada L, Juarez M (1998) Silver ion adsorption thin layer chromatography and capillary gas chromatography in the study of the composition of milk fat triglycerides. J Agric Food Chem 46:1836–1843

    Article  CAS  Google Scholar 

  34. Nikolova-Damyanova B (1999) Quantitative thin layer chromatography of triacylglycerols. Principles and application B. J Liq Chromatogr Rel Technol 22:1513–1537

    Article  CAS  Google Scholar 

  35. Flieger J, Szumilo H (2000) Optimizing chromatographic conditions for separation of fatty acid methyl esters by argentation thin layer chromatography. J Planar Chromatogr 13:426–431

    CAS  Google Scholar 

  36. Marevkov I, Tarandjiiska R, Panayotova S, Nikolova N (2001) Valuation of argentation TLC densitometry for the determination of trans fatty acids in butterfat. J Planar Chromatogr 14:384–390

    Google Scholar 

  37. Nikolova-Damyanova B, Velikova R, Kuleva L (2002) Quantitative TLC for determination of triacylglycerol composition of sesame seeds. J Liq Chromatogr Rel Technol 25:1623–1632

    Article  CAS  Google Scholar 

  38. Singh DK, Srivastava B, Sahu A (2003) Thin layer chromatography of purines on silica gel G impregnated with transition metal ions; assay of caffeine and theophylline in pharmaceutical formulations. J Chinese Chem Soc 50:1031–1036

    CAS  Google Scholar 

  39. Nimal Ratnayake WM (2004) Overview of methods for the determination of trans fatty acids by gas chromatography, silver-ion thin-layer chromatography, and gas chromatography/mass spectrometry. J Assoc Off Anal Chem 87:523–539

    Google Scholar 

  40. Cruz-hernandez C, Deng Z, Zhou J, Hill AR, Yurawecz MP, Delmonte P, Mossoba MM, Dugan MER, Kramer JKG (2004) Methods for the analysis of conjugated linoleic acids and trans-18:1 isomers in dairy fats by using a combination of gas chromatography, silver-ion thin-layer chromatography/gas chromatography, and silver ion liquid chromatography. J Assoc Off Anal Chem 87:545–562

    CAS  Google Scholar 

  41. Jarusiewicz J, Sherma J, Fried B (2005) Separation of sterols by reversed phase and argentation thin layer chromatography. Their identification in snail bodies. J Liq Chromatogr Rel Technol 28:2607–2617

    Article  CAS  Google Scholar 

  42. Destaillats F, Golay PA, Joffre F, De Wispelaere M, Hug B, Giuffrida F, Fauconnot L, Dionisi F (2007) Comparison of available analytical methods to measure trans-octadecenoic acid isomeric profile and content by gas-liquid chromatography in milk fat. J Chromatogr A 1145:222–228

    Article  CAS  Google Scholar 

  43. Mohammad A, Zehra A (2008) Separation of coexisting tryptophan, alanine, and phenylalanine or tyrosine by silver ion high performance thin layer chromatography. J Planar Chromatogr 21:299–304

    Article  CAS  Google Scholar 

  44. Srivastava SP, Reena (1985) TLC separation of some closely related sulfa drugs on copper sulfate impregnated plates. Anal Lett 18:239–244

    Google Scholar 

  45. Cecchi L, Malaspina P (1991) Lactic acid enantiomers: separation by thin-layer chromatography on silica gel plates impregnated with Cu2+. Anal Biochem 192:219–221

    Article  CAS  Google Scholar 

  46. Szumilo H, Flieger J (2001) TLC separation of carbohydrates on silica gel modified with copper (II) salts. Acta Pol Pharm─Drug Res 58:3–8

    CAS  Google Scholar 

  47. Bushan R, Gupta D (2006) Ligand-exchange TLC resolution of some beta-adrenergic blocking agents. J Planar Chromatogr 19:241–245

    Article  Google Scholar 

  48. Pyka A, Klimczok W (2008) Influence of impregnation of a mixture of silica gel and kieselguhr with copper (II) sulphate (VI) on profile change of the spectrodensitograms and the R F values of nicotinic acid and its derivatives. J Liq Chromatogr Rel Technol 31:526–542

    Article  CAS  Google Scholar 

  49. Bhushan R, Tanwar S (2009) Direct TLC resolution of the enantiomers of three β-blockers by ligand exchange with Cu(II)–L-amino acid complex, using four different approaches. Chromatographia 70:1001–1006

    Article  CAS  Google Scholar 

  50. Bhushan R, Tanwar S (2010) Different approaches of impregnation for resolution of enantiomers of atenolol, propranolol and salbutamol using Cu(II)-l-amino acid complexes for ligand exchange on commercial thin layer chromatographic plates. J Chromatogr A 1217:1395–1398

    Article  CAS  Google Scholar 

  51. Flieger J, Szumiło H, Giełzak-Koćwin K, Matosiuk D (2002) Effect of impregnation conditions on the structure and chromatographic behavior of TLC adsorbents modified with Cu(II) and Ni(II) salts. J Planar Chromatogr 15:354–360

    Article  Google Scholar 

  52. Petrovic’ M, Kaštelan-Macan M, Horvat AJM (1992) Thin-layer chromatographic behaviour of substituted phenolic compounds on silica gel layers impregnated with aluminium(III) and copper(II). J Chromatogr A 607:163–167

    Article  Google Scholar 

  53. Sober M, Lekic M, Korac B, Imamovic B, Marjanovic A (2003) Identification of chlorophenoxy herbicides by TLC in clinical toxicology. Proc Int Symp Planar Sep Plan Chrom 423–432

    Google Scholar 

  54. Pyka A, Dołowy M, Gurak D (2005) Separation of selected bile acids by TLC. VIII. Separation on silica gel 60F254 glass plates impregnated with Cu(II), Ni(II), Fe(II), and Mn(II) cations. J Liq Chromatogr Rel Technol 28:2273–2284

    Article  CAS  Google Scholar 

  55. Dołowy M (2007) Separation of selected bile acids by TLC. IX. Separation on silica gel 60 and on silica gel 60F254 aluminum plates impregnated with Cu(II), Ni(II), Fe(II), and Mn(II) cations. J Liq Chromatogr Rel Technol 30:405–418

    Article  Google Scholar 

  56. Bhushan R, Joshi S (1996) TLC separation of antihistamines on silica gel plates impregnated with transition metal ions. J Planar Chromatogr 9:70–72

    CAS  Google Scholar 

  57. Bhusan R, ThukuThiong O (2002) Separation of cephalosporins on thin silica gel layers impregnated with transition metal ions and by reversed-phase TLC. Biomed Chromatogr 16:165–174

    Article  Google Scholar 

  58. Grygierczyk G, Wasilewski J, Witkowska M, Kowalska T (2003) Use of complexation TLC to investigate selected monosulfides. Part I. Silica impregnated with Cu(II), Co(II), Ni(II), Mn(II), Al(III), Cr(III), and Fe(III) cations as stationary phase. J Planar Chromatogr 16:11–14

    Article  CAS  Google Scholar 

  59. Bhushan R, Parshad V (1994) Separation of vitamin B complex and folic acid using TLC plates impregnated with some transition metal ions. Biomed Chromatogr 8:196–198

    Article  CAS  Google Scholar 

  60. Bhushan R, Parshad V (1999) Improved separation of vitamin B complex and folic acid using some new solvent systems and impregnated TLC. J Liq Chromatogr Rel Technol 22:1607–1623

    Article  CAS  Google Scholar 

  61. Sajewicz M, John E, Kronenbach D, Gontarska M, Kowalska T (2008) TLC study of the separation of the enantiomers of lactic acid. Acta Chromatographica 20:367–382

    Article  CAS  Google Scholar 

  62. Kalinina KB, Litvinova LS (1999) Impregnation of silica gel with inorganic salts from the standpoint of specific adsorption theory. The effect of this impregnation on the separation of sugars by TLC. J Planar Chromatogr 12:190–195

    CAS  Google Scholar 

  63. Kalinina KB, Litvinova LS (2001) Thin layer chromatography of neutral sugars as influenced by the nature of the cation of impregnating salt. Russ J Appl Chem 74:1343–1347

    Article  CAS  Google Scholar 

  64. Wyss D, Esser T, Sequin U (1988) Chromatographic separation of diastereoisomeric 1,2:3,4-diepoxides on silica gel TLC plates impregnated with various inorganic salts. Tetrahedron 44:1393–1396

    Article  CAS  Google Scholar 

  65. Mohammad A, Agrawal V, Kumar S (2003) Use of a water-in-oil microemulsion as mobile phase in complexation TLC of amino acids on silica layers impregnated with metal cations. J Planar Chromatogr 16:220–226

    Article  CAS  Google Scholar 

  66. Bhushan R, Kaur S (1997) TLC separation of some common sugars on silica gel plates impregnated with transition metal ions. Biomed Chromatogr 11:59–60

    Article  CAS  Google Scholar 

  67. Bhushan R, Ali I (1989) TLC resolution of alkaloids on silica layers impregnated with metal ions. J Planar Chromatogr 2:397–398

    CAS  Google Scholar 

  68. Bhushan R, Ali I (1990) Adsorption and partition of amino acids in a batch process compared to their TLC separation on silica gel. J Planar Chromatogr 3:85–87

    CAS  Google Scholar 

  69. Bhushan R, Reddy GP (1987) Thin-layer chromatography of phenyl thiohydantoin derivatives of amino acids on silica gel thin layers impregnated with zinc salts and some metal sulphates. Analyst 112:1467–1469

    Article  CAS  Google Scholar 

  70. Flieger J, Szumilo H, Tatarczak M, Matosiuk D (2004) Effect of impregnation of silica gel with different zinc salts on the TLC behaviour of aromatic hydrocarbons with polar groups. J Planar Chromatogr 17:65–71

    Article  CAS  Google Scholar 

  71. Grygierczyk G, Wasilewski J, Łomankiewicz D, Klimczok W, Kowalska T (2003) Use of complexation TLC to investigate monosulfides. II. Silica impregnated with the Cd(II), Sr(II), Eu(III), and V(IV) cations as stationary phase. J Liq Chromatogr Rel Technol 26:2651–2661

    Article  CAS  Google Scholar 

  72. Bhushan R, Chauhan R, Chauhan RS (1989) Separation of some antihistamines on impregnated TLC silica plates. Biomed Chromatogr 3:46–47

    Article  CAS  Google Scholar 

  73. Bhushan R, Parshad V (1994) TLC of amino acids on thin silica layers impregnated with transition metal ions and their anions. J Planar Chromatogr 7:480–484

    CAS  Google Scholar 

  74. Fatima N, Mohammad A (1984) Thin layer chromatography of metal ions in formic acid medium on impregnated and unimpregnated silica Gel G: semiquantitative determination of Fe3+, Cd2+, Th4+, Al3+, Zn2+, UO 2+2 , VO2+, Ce4+ and Ni2+. Sep Sci Technol 19:429–443

    Article  Google Scholar 

  75. Ajmal M, Mohammad A, Fatima N, Ahmad J (1989) Aqueous alkali halides as impregnant and eluent in TLC of heavy metals: quantitative separation of Al, Ti, Fe, Zn and Cd. J Liq Chromatogr Rel Technol 12:3163–3191

    CAS  Google Scholar 

  76. Mohammad A, Khan MAM (1993) New surface-modified sorbent layer for the analysis of toxic metals in sea water and industrial wastewater. J Chromatogr A 642:455–458

    Article  CAS  Google Scholar 

  77. Mohammad A, Khan MAM (1995) Thin layer chromatographic separation of zinc from Cd(ll), Hg(ll), and Ni(ll) in environmental samples using impregnated silica layers. J Chromatogr Sci 33:531–535

    CAS  Google Scholar 

  78. Mohammad A, Fatima N, Khan MAM (1994) New surface-modified sorbent layers for chromatographic analysis of some inorganic pollutants. J Planar Chromatogr 7:142–146

    CAS  Google Scholar 

  79. Mohammad A, Khan MAM (1995) TLC separation and microgram detection of metal ions on lithium chloride-impregnated silica gel and alumina layers. J Planar Chromatogr 8:134–140

    CAS  Google Scholar 

  80. Takedaa Y, Ishidaa K, Hasegawab T, Katohc A (2004) Thin layer chromatographic behavior and separation of alkaline earth metals on silica gel in aqueous sodium perchlorate solution. J Chromatogr A 1049:233–236

    Google Scholar 

  81. Mohammad A, Shahab H (2008) Identification and simultaneous separation of manganese(II), copper(II) and nickel(II) on sodium hydroxide impregnated silica layers using water and aqueous surfactants as eluents. Acta Universitatis Cibiniensis Seria F Chemia 11:3–14

    Google Scholar 

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Acknowledgment

One of the authors (Abdul Moheman) is thankful to the Council of Scientific and Industrial Research (CSIR) of New Delhi, India, for providing financial assistance. They thank the Deanship of Scientific Research at King Saud University for funding the work through the research group project No. RGP-VPP-130.

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Authors and Affiliations

  1. Department of Applied Chemistry, Aligarh Muslim University, Aligarh, 202 002, India

    Ali Mohammad

  2. Department of Chemistry, Aligarh Muslim University, Aligarh, 202 002, India

    Abdul Moheman

  3. Department of Chemistry, College of Science, King Saud University, Riyadh, Saudi Arabia

    Gaber E. El-Desoky

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  1. Ali Mohammad
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  3. Gaber E. El-Desoky
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Correspondence to Ali Mohammad .

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Editors and Affiliations

  1. Faculty of Engineering & Technology, Department of Applied Chemistry, Aligarh Muslim University, Aligarh, 202 002, India

    Dr. Inamuddin

  2. College of Engineering, Chemical Engineering Department, King Saud University, Riyadh, Saudi Arabia

    Mohammad Luqman

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Mohammad, A., Moheman, A., El-Desoky, G.E. (2012). Cation-Exchanged Silica Gel–Based Thin-Layer Chromatography of Organic and Inorganic Compounds. In: Inamuddin, D., Luqman, M. (eds) Ion Exchange Technology II. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4026-6_16

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