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Separation of Amino Acids, Peptides, and Proteins by Ion Exchange Chromatography

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Book cover Ion Exchange Technology II

Abstract

Separation of amino acids, peptides, and proteins (bioanalytes) via ion exchange (IE) has widespread usage because it is usually very simple to design and it has high capacity and easily achievable control of the separation process. Amino acids, as principal constituents of proteins and having a plethora of biological functions of their own, are always in focus when developing novel methods. Separation and quantification of amino acids is essential in food science, medicine, agricultural science, etc. Peptides exist in nature and have diverse functions. Digestion of proteins by enzymes also gives complex mixtures of peptides and IE finds its application in peptide separation. There are lots of reasons for the popularity of IE in protein isolation and purification. It is used in research, analysis, and large-scale purification of proteins. Ion exchange is ideal for the initial capture of proteins because of its high capacity, relatively low cost, and its ability to survive rigorous cleaning regimes. This chapter covers basic principles and modern applications of IE in separation of amino acids, peptides, and proteins.

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Acknowledgments

The authors acknowledge the support of the Ministry of Science and Technological Development of the Republic of Serbia (Grant No. 172024).

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  1. University of Belgrade, Studentski Trg 12-16, 11000, Belgrade, Serbia

    Tanja Cirkovic Velickovic, Jana Ognjenovic & Luka Mihajlovic

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  1. Tanja Cirkovic Velickovic
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  2. Jana Ognjenovic
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Correspondence to Tanja Cirkovic Velickovic .

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Editors and Affiliations

  1. Faculty of Engineering & Technology, Department of Applied Chemistry, Aligarh Muslim University, Aligarh, 202 002, India

    Dr. Inamuddin

  2. College of Engineering, Chemical Engineering Department, King Saud University, Riyadh, Saudi Arabia

    Mohammad Luqman

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Velickovic, T.C., Ognjenovic, J., Mihajlovic, L. (2012). Separation of Amino Acids, Peptides, and Proteins by Ion Exchange Chromatography. In: Inamuddin, D., Luqman, M. (eds) Ion Exchange Technology II. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4026-6_1

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