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Cell Internalizing Anti-mortalin Antibody as a Nanocarrier

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Mortalin Biology: Life, Stress and Death

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Abstract

Nanocarriers are the small molecules with capability of adhering to or pass through the cell membrane and deliver the genes, peptides, growth factors or toxins to living cells. They form an extremely important part of gene delivery and molecular therapeutics. Besides the viral modes of gene delivery, cationic carrier molecules (e.g., liposome and polymers) that form non-covalent complexes with DNA constitute a major class of nanocarriers. Although relatively less efficient than viral systems, cationic carrier molecules have inherent advantage of flexibility and safety. Their derivatives in conjugation with functional molecules such as, peptides, proteins, growth factors and antibodies have been in focus to generate nanocarriers with low toxicity, high stability, high efficiency and cell-specific targeting features. In this chapter, we discuss the use of cell internalizing antibodies against mortalin protein as nanocarriers. Internalizing anti-mortalin antibodies was employed for (i) internalization of nanoparticles (quantum dots, QD) to generate illuminating cells and (ii) gene delivery. Furthermore, cationic polymer polyethylenimine (PEI) and internalizing anti-mortalin antibody complex was shown to enhance the gene delivery specifically to cancer cells and thus enabling the latter to serve as a novel cancer-targeting nanocarrier.

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Authors and Affiliations

  1. Center for Childhood Cancer, The Research Institute at Nationwide Children’s Hospital, Columbus, Ohio, USA

    Zeenia Kaul

  2. National Institute of Advanced Industrial Science and Technology (AIST), Central 4, 1-1-1 Higashi, Ibaraki 305-8562, Tsukuba, Japan

    Tomoko Yaguchi, Renu Wadhwa & Sunil C. Kaul

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  1. Zeenia Kaul
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  2. Tomoko Yaguchi
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  3. Renu Wadhwa
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  4. Sunil C. Kaul
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Correspondence to Sunil C. Kaul .

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Editors and Affiliations

  1. National Institute of Advanced Industria, Central 4, Higashi 1 -1-1, Tsukuba, 305 8566, Ibaraki, Japan

    Sunil C. Kaul

  2. Science & Technology (AIST), National Insitute of Advanced Industrial, Central 4, Higashi 1-1-1, Tsukuba, 305-8562, Ibaraki, Japan

    Renu Wadhwa

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Kaul, Z., Yaguchi, T., Wadhwa, R., Kaul, S.C. (2012). Cell Internalizing Anti-mortalin Antibody as a Nanocarrier. In: Kaul, S., Wadhwa, R. (eds) Mortalin Biology: Life, Stress and Death. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-3027-4_21

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