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Stem Cells and Cancer Stem Cells, Volume 6 pp 319–331Cite as

Migration of Stem Cells: Role of the RhoA/ROCK I Pathway (Method)

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Part of the book series: Stem Cells and Cancer Stem Cells ((STEM,volume 6))

Abstract

Cell migration is an important biological phenomenon that has come under the spotlight following the worldwide emergence of stem cell-based therapies. How a given stem cell migrates within an organism to reach its final destination, known as the stem cell niche, to replenish a cellular system is a question of interest. The development of new cell-isolation and transfection techniques together with ex vivo culture systems have allowed the successful isolation, manipulation and expansion of stem cells. When combined with real-time imaging techniques, novel biochemical tools such as RNA interference oligonucleotides and animal models, have shed new light on the mechanisms that regulate stem cell migration. Here, we summarize the migration mechanisms that are based on biochemical pathways related to Rho GTPases. In particular, we focus on the RhoA/ROCK I pathway that affects the polarization of hematopoietic stem and progenitor cells and therefore the driving forces underlying migration.

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Acknowledgement

The study was supported by Deutsche Forschungsgemeinschaft (TRR83 No. 6, SFB655 B3 and CO298/5-1).

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Authors and Affiliations

  1. Tissue Engineering Laboratories, Technische Universität Dresden, Tatzberg 47-49, D-01307, Dresden, Germany

    Ana-Violeta Fonseca, Doreen Reichert & Denis Corbeil

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  1. Ana-Violeta Fonseca
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  2. Doreen Reichert
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  3. Denis Corbeil
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Correspondence to Denis Corbeil .

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  1. , Kean University, Room 213, Library building, Morris Avenue 1000, Union, 07083, New Jersey, USA

    M.A. Hayat

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Fonseca, AV., Reichert, D., Corbeil, D. (2012). Migration of Stem Cells: Role of the RhoA/ROCK I Pathway (Method). In: Hayat, M. (eds) Stem Cells and Cancer Stem Cells, Volume 6. Stem Cells and Cancer Stem Cells, vol 6. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-2993-3_28

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