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Metabolite Profile Differences in Childhood Brain Tumors: 1H Magic Angle Spinning NMR Spectroscopy

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Pediatric Cancer, Volume 2

Part of the book series: Pediatric Cancer ((PECA,volume 2))

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Abstract

Background: Brain and nervous system tumours are the most common solid cancers in children. Molecular characterisation of these tumours is important for providing novel biomarkers of disease and identifying molecular pathways which may provide putative targets for new therapies. 1H High Resolution Magic Angle Spinning NMR spectroscopy (HR-MAS) is an emerging technique for determining metabolite profiles from small pieces of intact tissue and whole cells grown in culture, making it ideal for molecular characterisation of disease.

Method: 22 tissue samples from children with brain tumours and 7 cell lines originating from medulloblastoma (2), neuroblastoma (2), supertentorial PNET (1) and retinoblastoma (2) were analysed using HR-MAS. Spectra were fitted to a library of individual metabolite spectra to provide metabolite values, Principle Component Analysis (PCA) was used to investigate the metabolic relationship between the tumour types.

Results: Primitive neuroectodermal and glial based tumours were linearly separable using PCA, demonstrating that histological features of the tissue were closely related to their metabolite profiles. Each cultured cell line was found to have a distinct metabolite profile. The desmoplastic and classic variants of medulloblastoma were particularly distinct, with large differences in the distribution of choline containing metabolites.

Conclusion: HR-MAS identified key differences in the metabolite profiles of childhood brain tumour tissue and cells grown in culture, improving the molecular characterisation of these tumours and showing the promise of HR-MAS as a rapid diagnostic aid. Further investigation of the underlying molecular pathways is required to assess their potential as targets for new agents.

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Authors and Affiliations

  1. Cancer Sciences, University of Birmingham, Birmingham, UK

    Martin Wilson & Andrew Peet

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  1. Martin Wilson
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  2. Andrew Peet
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Correspondence to Martin Wilson .

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  1. , Kean University, Room 213, Library building, Morris Avenue 1000, Union, 07083, New Jersey, USA

    M.A. Hayat

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Wilson, M., Peet, A. (2012). Metabolite Profile Differences in Childhood Brain Tumors: 1H Magic Angle Spinning NMR Spectroscopy. In: Hayat, M. (eds) Pediatric Cancer, Volume 2. Pediatric Cancer, vol 2. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-2957-5_11

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