Abstract
Heterotrophic model protocells are easier to build in the laboratory than autotrophic protocells. Advancements have shown that model heterotrophic systems can grow, divide, copy nucleic acids, and compete for resources. The required component molecules of the system are few and simple, in the sense that only a small number of monomeric species are needed. Polymerization and noncovalent forces under prebiotically plausible conditions result in the assembly of a system that begins to exhibit life-like properties. The data suggest that similar processes may have occurred on prebiotic Earth.
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References
Apel CL, Deamer DW (2005) The formation of glycerol monodecanoate by a dehydration condensation reaction: increasing the chemical complexity of amphiphiles on the early earth. Orig Life Evol Biosph 35:323–332
Baaske P, Weinert FM, Duhr S, Lemke KH, Russel MJ, Braun D (2007) Extreme accumulation of nucleotides in simulated hydrothermal pore systems. Proc Natl Acad Sci USA 104:9346–9351
Biondi E, Branciamore S, Maurel MC, Gallori E (2007) Montmorillonite protection of an UV-irradiated hairpin ribozyme: evolution of the RNA world in a mineral environment. BMC Evol Biol 7(Suppl 2):S2
Budin I, Szostak JW (2010) Expanding roles for diverse physical phenomena during the origin of life. Annu Rev Biophys 39:245–263
Budin I, Bruckner RJ, Szostak JW (2009) Formation of protocell-like vesicles in a thermal diffusion column. J Am Chem Soc 131:9628–9629
Chen IA, Szostak JW (2004a) A kinetic study of the growth of fatty acid vesicles. Biophys J 87:988–998
Chen IA, Szostak JW (2004b) Membrane growth can generate a transmembrane pH gradient in fatty acid vesicles. Proc Natl Acad Sci USA 101:7965–7970
Chen IA, Roberts RW, Szostak JW (2004) The emergence of competition between model protocells. Science 305:1474–1476
Chen IA, Salehi-Ashtiani K, Szostak JW (2005) RNA catalysis in model protocell vesicles. J Am Chem Soc 127:13213–13219
Deamer DW (1997) The first living systems: a bioenergetic perspective. Microbiol Mol Biol Rev 61:239–261
Ferris JP, Ertem G, Agarwal VK (1989) The adsorption of nucleotides and polynucleotides on montmorillonite clay. Orig Life Evol Biosph 19:153–164
Ferris JP, Hill AR, Liu R, Orgel LE (1996) Synthesis of long prebiotic oligomers on mineral surfaces. Nature 381:59–61
Gebicki JM, Hicks M (1973) Ufasomes are stable particles surrounded by unsaturated fatty acid membranes. Nature 243:232–234
Gebicki JM, Hicks M (1976) Preparation and properties of vesicles enclosed by fatty acid membranes. Chem Phys Lipids 16:142–146
Gibson DG, Glass JI, Lartigue C, Noskov VN, Chuang RY, Algire MA, Benders GA, Montague MG, Ma L, Moodie MM et al (2010) Creation of a bacterial cell controlled by a chemically synthesized genome. Science 329:52–56
Gil R, Silva FJ, Pereto J, Moya A (2004) Determination of the core of a minimal bacterial gene set. Microbiol Mol Biol Rev 68:518–537
Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC (2006) Essential genes of a minimal bacterium. Proc Natl Acad Sci USA 103:425–430
Hanczyc MM, Fujikawa SM, Szostak JW (2003) Experimental models of primitive cellular compartments: encapsulation, growth, and division. Science 302:618–622
Hanczyc MM, Mansy SS, Szostak JW (2007) Mineral surface directed membrane assembly. Orig Life Evol Biosph 37:67–82
Hargreaves WR, Deamer DW (1978) Liposomes from ionic, single-chain amphiphiles. Biochemistry 17:3759–3768
Hargreaves WR, Mulvihill SJ, Deamer DW (1977) Synthesis of phospholipids and membranes in prebiotic conditions. Nature 266:78–80
Kuo CH, Ochman H (2009) Deletional bias across the three domains of life. Genome Biol Evol 1:145–152
Lohrmann R (1977) Formation of nucleoside 5′-phosphoramidates under potentially prebiological conditions. J Mol Evol 10:137–154
Lohrmann R, Bridson PK, Orgel LE (1980) Efficient metal-ion catalyzed template-directed oligonucleotide synthesis. Science 208:1464–1465
Lorsch JR, Szostak JW (1996) Chance and necessity in the selection of nucleic acid catalysts. Acc Chem Res 29:103–110
Luisi PL (2003) Autopoiesis: a review and a reappraisal. Naturwissenschaften 90:49–59
Mansy SS (2010) Membrane transport in primitive cells. Cold Spring Harb Perspect Biol 2:a002188
Mansy SS, Szostak JW (2008) Thermostability of model protocell membranes. Proc Natl Acad Sci USA 105:13351–13355
Mansy SS, Szostak JW (2009) Reconstructing the emergence of cellular life through the synthesis of model protocells. Cold Spring Harb Symp Quant Biol 74:47–54
Mansy SS, Schrum JP, Krishnamurthy M, Tobe S, Treco DA, Szostak JW (2008) Template-directed synthesis of a genetic polymer in a model protocell. Nature 454:122–125
Maurer SE, Deamer DW, Boncella JM, Monnard PA (2009) Chemical evolution of amphiphiles: glycerol monoacyl derivatives stabilize plausible prebiotic membranes. Astrobiology 9:979–987
McCollom TM, Ritter G, Simoneit BR (1999) Lipid synthesis under hydrothermal conditiosn by Fischer-Tropsch-type reactions. Orig Life Evol Biosph 29:153–166
McCutcheon JP, Moran NA (2007) Parallel genomic evolution and metabolic interdependence in an ancient symbiosis. Proc Natl Acad Sci USA 104:19392–19397
Miller SL (1953) A production of amino acids under possible primitive earth conditions. Science 117:528–529
Monnard PA, Apel CL, Kanavarioti A, Deamer DW (2002) Influence of ionic inorganic solutes on self-assembly and polymerization processes related to early forms of life: implications for a prebiotic aqueous medium. Astrobiology 2:139–152
Monnard PA, Kanavarioti A, Deamer DW (2003) Eutectic phase polymerization of activated ribonucleotide mixtures yields quasi-equimolar incorporation of purine and pyrimidine nucleobases. J Am Chem Soc 125:13734–13740
Morowitz HJ (1992) Beginnings of cellular life. Metabolism recapitulates biogenesis. Yale University Press, New Haven
Oparin AI (2003) The origin of life, 2nd edn. Dover Publications, Inc, Mineola
Oro J (1961) Mechanism of synthesis of adenine from hydrogen cyanide under possible primitive earth conditions. Nature 191:1193–1194
Pizzarello S, Shock E (2010) The organic composition of carbonaceous meteorites: the evolutionary story ahead of biochemistry. Cold Spring Harb Perspect Biol 2:a002105
Powner MW, Gerland B, Sutherland JD (2009) Synthesis of activated pyrimidine ribonucleotides in prebiotically plausible conditions. Nature 459:239–242
Rajamani S, Ichida JK, Antal T, Treco DA, Leu K, Nowak MA, Szostak JW, Chen IA (2010) Effect of stalling after mismatches on the error catastrophe in nonenzymatic nucleic acid replication. J Am Chem Soc 132:5880–5885
Ricardo A, Carrigan MA, Olcott AN, Benner SA (2004) Borate minerals stabilize ribose. Science 303:196
Schrum JP, Ricardo A, Krishnamurthy M, Blain JC, Szostak JW (2009) Efficient and rapid template-directed nucleic acid copying using 2′-amino-2′,3′-dideoxyribonucleoside-5′-phosphorimidazolide monomers. J Am Chem Soc 131:14560–14570
Shew RL, Deamer DW (1985) A novel method for encapsulation of macromolecules in liposomes. Biochim Biophys Acta 816:1–8
Sleeper HL, Orgel LE (1979) The catalysis of nucleotide polymerization by compounds of divalent lead. J Mol Evol 12:357–364
Szostak JW, Bartel DP, Luisi PL (2001) Synthesizing life. Nature 409:387–390
Takahashi H, Kageyama Y, Kurihara K, Takakura K, Murata S, Sugawara T (2010) Autocatalytic membrane-amplification on a pre-existing vesicular surface. Chem Commun 46:8791–8793
Walde P (2006) Surfactant assemblies and their varioius possible roles for the origin(s) of life. Orig Life Evol Biosph 36:109–150
Woese C (1998) The universal ancestor. Proc Natl Acad Sci USA 95:6854–6859
Zepik HH, Blochliger E, Luisi PL (2001) A chemical model of homeostasis. Angew Chem Int Ed Engl 40:199–202
Zhu TF, Szostak JW (2009) Coupled growth and division of model protocell membranes. J Am Chem Soc 131:5705–5713
Zielinski WS, Orgel LE (1985) Oligomerization of activated derivatives of 3′-amino-3′-deoxyguanosine on poly(C) and poly(dC) templates. Nucleic Acids Res 13:2469–2484
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Del Bianco, C., Mansy, S.S. (2012). Heterotrophic Model Protocells. In: Seckbach, J. (eds) Genesis - In The Beginning. Cellular Origin, Life in Extreme Habitats and Astrobiology, vol 22. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-2941-4_35
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DOI: https://doi.org/10.1007/978-94-007-2941-4_35
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