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Techniques and Methodologies to Study the Ryanodine Receptor at the Molecular, Subcellular and Cellular Level

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Calcium Signaling

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 740))

Abstract

In excitable tissues, the ryanodine receptor Ca2+ release channel (RyR) protein complex regulates excitation-contraction coupling, exocytosis, gene expression and apoptosis. Defects in RyR function, in genetic or acquired pathologies, lead to massive disruptions of Ca2+ release that can be lethal. Therefore, RyR has emerged as a putative therapeutic target and an increasing number of RyR-targeting drugs are currently being tested.

Nonetheless this large-size channel is still a mystery in terms of structure, which hinders full characterization of the properties of this central protein. This chapter is dedicated to the methods available to examine RyR structure and function. The aim of the article is to concentrate on contemporary methodologies rather than focusing overtly on the progress that has been achieved using these techniques. Here we review a series of reliable approaches that are routinely employed to investigate this channel. Technical limitations are discussed, and technological developments are presented. This work is not a handbook, but it can be used as a resource and a starting point for the investigation of RyR at different levels of resolution.

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Acknowledgements

We are very much obliged to the British Heart Foundation, which provides the financial support for all researchers involved in this work. Moreover we would like to thank Prof. Peter Lipp and Dr. Lars Kaestner from the Institute of Molecular Cell Biology (Medical Faculty, Saarland University, Homburg/Saar, Germany) for their great support and help in monitoring Ca2+ dynamics in rat heart muscle cells and the use of confocal microscopy.

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  1. Department of Cardiology, Wales Heart Research Institute, School of Medicine, Cardiff University, Heath Park, Cardiff, CF14 4XN, UK

    Cedric Viero, N. Lowri Thomas, Joanne Euden, Sammy A. Mason, Christopher H. George & Alan J. Williams

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  1. Cedric Viero
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  2. N. Lowri Thomas
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  3. Joanne Euden
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Correspondence to Cedric Viero .

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  1. , Department of Clinical Sciences and Educ, Karolinska Institutet, Tulegatan 26, Stockholm, SE-118 83, Sweden

    Md. Shahidul Islam

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Viero, C., Thomas, N.L., Euden, J., Mason, S.A., George, C.H., Williams, A.J. (2012). Techniques and Methodologies to Study the Ryanodine Receptor at the Molecular, Subcellular and Cellular Level. In: Islam, M. (eds) Calcium Signaling. Advances in Experimental Medicine and Biology, vol 740. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-2888-2_8

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