Locomotor Recovery After Spinal Cord Transection: Transplantation of Oligodendrocytes and Motoneuron Progenitors Generated from Human Embryonic Stem Cells
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Currently there is no cure for spinal cord injury (SCI). A large number of different cell types have been tested for possible cell treatment therapy, with different results in locomotor and sensory improvement. Human embryonic stem cells (hESC) hold great promise for the treatment of patients with many neurodegenerative diseases particularly those arising from cell loss or neural dysfunction including SCI. Here we evaluate the therapeutic effects of transplanted hESC-derived oligodendrocyte progenitors (OPC) and/or motoneuron progenitors (MP) on axonal remyelination and functional recovery of adult rats after complete spinal cord transection. OPC and/or MP were grafted into the site of injury in the acute phase. Based on Basso-Beattie-Bresnahan scores recovery of locomotor function was significantly enhanced in rats treated with OPC and/or MP when compared with control animals. When transplanted into the spinal cord immediately after complete transection OPC and MP survived, migrated and differentiated into mature oligodendrocytes and neurons showing in vivo electrophysiological activity. Taken together, these indicate that OPC and MP derived from hESC could be a useful therapeutic strategy to repair injured spinal cord.
KeywordsSpinal Cord Injury Human Embryonic Stem Cell Spinal Motoneuron Spinal Cord Transection Mature Oligodendrocyte
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