Biological Decontamination Using Pulsed Filamentary Microplasma Jet

  • Ramasamy PothirajaEmail author
  • Jan-Wilm Lackmann
  • Gernot Keil
  • Nikita Bibinov
  • Peter Awakowicz
Conference paper
Part of the NATO Science for Peace and Security Series A: Chemistry and Biology book series (NAPSA)


Microplasma jet for the generation of pulsed filamentary discharge at atmospheric pressure has been devised for biological decontamination as well as for modification of surface properties. Long plasma-filament is generated inside a quartz tube and characterized using optical emission spectroscopy, current voltage measurements, numerical simulations and microphotography. Efficiency of our plasma source for the decontamination on inner surface of the tube as well as on objects placed in proximity of plasma effluent is studied. Escherichia coli (Gram-negative bacteria) and spores of Bacillus atrophaeus (Gram-positive bacteria) are used for the decontamination studies. Decontamination of Bacillus atrophaeus endospores, which are layered on PET polymer material, and placed in the proximity of plasma effluent, shows the mean logarithmic bacterial reduction of 3.67 for the treatment time of 120 s. Inactivation of Escherichia coli coated on inner surface of the tube shows the mean logarithmic bacterial reduction of about 5 for the treatment time of 30 s. In addition to this, inhibition studies of bacteria coated on agar plate are also carried out. It shows plasma effluent generated in our plasma source is very effective for the inhibition of bacterial colonization.


Plasma Source Rotational Temperature Electron Impact Excitation Electron Velocity Distribution Function Current Voltage Measurement 
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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Ramasamy Pothiraja
    • 1
    Email author
  • Jan-Wilm Lackmann
    • 2
  • Gernot Keil
    • 3
  • Nikita Bibinov
    • 1
  • Peter Awakowicz
    • 1
  1. 1.Institute for Electrical Engineering and Plasma TechnologyRuhr-Universität BochumBochumGermany
  2. 2.Microbial Biology, Department for Biology and BiotechnologyRuhr University BochumBochumGermany
  3. 3.KHS GmbHBad KreuznachGermany

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