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Biological Decontamination Using Pulsed Filamentary Microplasma Jet

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Plasma for Bio-Decontamination, Medicine and Food Security

Abstract

Microplasma jet for the generation of pulsed filamentary discharge at atmospheric pressure has been devised for biological decontamination as well as for modification of surface properties. Long plasma-filament is generated inside a quartz tube and characterized using optical emission spectroscopy, current voltage measurements, numerical simulations and microphotography. Efficiency of our plasma source for the decontamination on inner surface of the tube as well as on objects placed in proximity of plasma effluent is studied. Escherichia coli (Gram-negative bacteria) and spores of Bacillus atrophaeus (Gram-positive bacteria) are used for the decontamination studies. Decontamination of Bacillus atrophaeus endospores, which are layered on PET polymer material, and placed in the proximity of plasma effluent, shows the mean logarithmic bacterial reduction of 3.67 for the treatment time of 120 s. Inactivation of Escherichia coli coated on inner surface of the tube shows the mean logarithmic bacterial reduction of about 5 for the treatment time of 30 s. In addition to this, inhibition studies of bacteria coated on agar plate are also carried out. It shows plasma effluent generated in our plasma source is very effective for the inhibition of bacterial colonization.

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Author information

Authors and Affiliations

  1. Institute for Electrical Engineering and Plasma Technology, Ruhr-Universität Bochum, 44801, Bochum, Germany

    Ramasamy Pothiraja, Nikita Bibinov & Peter Awakowicz

  2. Microbial Biology, Department for Biology and Biotechnology, Ruhr University Bochum, Universitätsstr. 150, 44801, Bochum, Germany

    Jan-Wilm Lackmann

  3. KHS GmbH, 55543, Bad Kreuznach, Germany

    Gernot Keil

Authors
  1. Ramasamy Pothiraja
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  2. Jan-Wilm Lackmann
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  3. Gernot Keil
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  4. Nikita Bibinov
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  5. Peter Awakowicz
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Corresponding author

Correspondence to Ramasamy Pothiraja .

Editor information

Editors and Affiliations

  1. , Faculty of Math., Phys. and Informatics, Comenius University, Mlynska Dolina, Bratislava, 84248, Slovakia

    Zdenko Machala

  2. , Faculty of Math., Phys. and Informatics, Comenius University, Mlynska Dolina, Bratislava, 84248, Slovakia

    Karol Hensel

  3. SRC RF Triniti, Pushkovykh 12, Troitsk, 142190, Russia

    Yuri Akishev

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© 2012 Springer Science+Business Media B.V.

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Pothiraja, R., Lackmann, JW., Keil, G., Bibinov, N., Awakowicz, P. (2012). Biological Decontamination Using Pulsed Filamentary Microplasma Jet. In: Machala, Z., Hensel, K., Akishev, Y. (eds) Plasma for Bio-Decontamination, Medicine and Food Security. NATO Science for Peace and Security Series A: Chemistry and Biology. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-2852-3_4

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