Amino Acid Homeostasis and Chronological Longevity in Saccharomyces cerevisiae

  • John P. ArisEmail author
  • Laura K. Fishwick
  • Michelle L. Marraffini
  • Arnold Y. Seo
  • Christiaan Leeuwenburgh
  • William A. DunnJr.
Part of the Subcellular Biochemistry book series (SCBI, volume 57)


Understanding how non-dividing cells remain viable over long periods of time, which may be decades in humans, is of central importance in understanding mechanisms of aging and longevity. The long-term viability of non-dividing cells, known as chronological longevity, relies on cellular processes that degrade old components and replace them with new ones. Key among these processes is amino acid homeostasis. Amino acid homeostasis requires three principal functions: amino acid uptake, de novo synthesis, and recycling. Autophagy plays a key role in recycling amino acids and other metabolic building blocks, while at the same time removing damaged cellular components such as mitochondria and other organelles. Regulation of amino acid homeostasis and autophagy is accomplished by a complex web of pathways that interact because of the functional overlap at the level of recycling. It is becoming increasingly clear that amino acid homeostasis and autophagy play important roles in chronological longevity in yeast and higher organisms. Our goal in this chapter is to focus on mechanisms and pathways that link amino acid homeostasis, autophagy, and chronological longevity in yeast, and explore their relevance to aging and longevity in higher eukaryotes.


Amino acid Homeostasis Chronological longevity Caloric restriction Autophagy 



branched side-chain amino acids


chronological life span


calorie restriction


general amino acid control


iron sulfur cluster


nitrogen catabolite repression


reactive oxygen species


target of rapamycin



We are grateful for the support that we have received from the NIH (AG023719 to JPA; AG17994 to CL; CA95552 to WAD), including the Claude D. Pepper Older Americans Independence Center (AG028740), and the University of Florida Institute on Aging. We acknowledge the Honors Program at the University of Florida, which has facilitated the participation of undergraduate students in our research efforts.


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • John P. Aris
    • 1
    Email author
  • Laura K. Fishwick
    • 1
  • Michelle L. Marraffini
    • 1
  • Arnold Y. Seo
    • 1
  • Christiaan Leeuwenburgh
    • 2
  • William A. DunnJr.
    • 1
  1. 1.Department of Anatomy and Cell BiologyUniversity of FloridaGainesvilleUSA
  2. 2.Department of Aging and Geriatric ResearchUniversity of FloridaGainesvilleUSA

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