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Exploring the Mechanism of Biomolecule Immobilization on Plasma-Treated Polymer Substrates

Biomolecule Immobilization on Plasma-Treated Polymer

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Technological Innovations in Sensing and Detection of Chemical, Biological, Radiological, Nuclear Threats and Ecological Terrorism

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Abstract

An electron beam-generated plasma source developed at NRL was used to modify polystyrene microtitre plates. A combination of complementary surface analytical and biochemical techniques was applied to evaluate the relationship between the biotic and abiotic layers, with particular emphasis on the efficiency of the preparation of polymer surfaces and its effectiveness for bioimmobilization. We conclude that the development of novel interface materials with superior transducing capabilities is dependent on the deeper understanding of the complex physicochemical, nanoscale interactions between the substrate surface and the biological components attached to it.

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Acknowledgements

J. W. is recipient of an American Society of Engineering Education postdoctoral fellowship. This work is funded by Joint Science & Technology Office for Chemical & Biological Defense/Defense Threat Reduction Agency and the Office of Naval Research. The views expressed here are those of the authors and do not represent the opinions of the U.S. Navy, the U.S. Department of Defense, or the U.S. government.

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Authors and Affiliations

  1. Plasma Physics Division, U.S. Naval Research Laboratory, 4555 Overlook Ave. SW, Washington, DC, USA

    E. H. Lock & S. G. Walton

  2. Center for Bio/Molecular Science & Engineering, U.S. Naval Research Laboratory, Washington, DC, USA

    S. H. North, J. Wojciechowski & C. R. Taitt

Authors
  1. E. H. Lock
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  2. S. H. North
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  3. J. Wojciechowski
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  4. C. R. Taitt
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  5. S. G. Walton
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Corresponding author

Correspondence to E. H. Lock .

Editor information

Editors and Affiliations

  1. , NUARI, Institute for Advanced Sciences Converge, 13873 Park Center Rd, Suite 500, Herndon, 20171, Virginia, USA

    Ashok Vaseashta

  2. Norwich University Applied Research Inst, 158 Harmon Dr., Northfield, 05663, Vermont, USA

    Eric Braman

  3. Norwich University Applied Research Inst, 158 Harmon Dr., Northfield, 05663, Vermont, USA

    Philip Susmann

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© 2012 Springer Science+Business Media B.V.

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Lock, E.H., North, S.H., Wojciechowski, J., Taitt, C.R., Walton, S.G. (2012). Exploring the Mechanism of Biomolecule Immobilization on Plasma-Treated Polymer Substrates. In: Vaseashta, A., Braman, E., Susmann, P. (eds) Technological Innovations in Sensing and Detection of Chemical, Biological, Radiological, Nuclear Threats and Ecological Terrorism. NATO Science for Peace and Security Series A: Chemistry and Biology. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-2488-4_26

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