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Tumors of the Central Nervous System, Volume 5 pp 73–79Cite as

Development of Cysts in Pilocytic Astrocytomas: Role  of Eosinophilic Granular Bodies (Method)

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Part of the book series: Tumors of the Central Nervous System ((TCNS,volume 5))

Abstract

Pilocytic astrocytomas are the major cystic astrocytoma found in childhood central nervous system tumor. Eosinophilic granular bodies (EGBs) are ground eosinophilic hyaline bodies observed in microcysts in pilocytic astrocytomas. EGBs have long been thought to be related to the lysosomal system. Lysosomes and their proteases play important roles in the progression of various cancers including the brain tumors. However, the presence of lysosomal proteins in EGBs in pilocytic astrocytomas and their relationship to disease progression are not fully studied. We recently showed that EGBs can be observed by immunohistochemistry with antibodies against the lysosomal membrane proteins, LAMP-1 and LAMP-2, and the major lysosomal protease, cathepsin D. Eosinophilic granular bodies, together with other factors, may be involved in the development of cysts in pilocytic astrocytomas. Here we discuss the possible role of EGBs in cyst development in pilocytic astrocytomas. EGBs observation facilitates brain tumor discrimination; we also provide protocol for EGBs observation using immunohistochemistry with antibodies against LAMP-1 and LAMP-2. LAMP-1 and LAMP-2 can show distinct ring-like pattern in EGBs staining and this facilitates EGBs observation.

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Authors and Affiliations

  1. Section of Haematology-Oncology, Department of Medicine, Taipei Medical University and Hospital, Taipei, 110, Taiwan

    Jai-Nien Tung, Tang-Yi Tsao, Kun-Tu Yeh, Ching-Fong Liao & Ming-Chung Jiang

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  1. Jai-Nien Tung
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  2. Tang-Yi Tsao
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  3. Kun-Tu Yeh
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  4. Ching-Fong Liao
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  5. Ming-Chung Jiang
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Correspondence to Ming-Chung Jiang .

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  1. , Kean University, Room 213, Library building, Morris Avenue 1000, Union, 07083, New Jersey, USA

    M.A. Hayat

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Tung, JN., Tsao, TY., Yeh, KT., Liao, CF., Jiang, MC. (2012). Development of Cysts in Pilocytic Astrocytomas: Role  of Eosinophilic Granular Bodies (Method). In: Hayat, M. (eds) Tumors of the Central Nervous System, Volume 5. Tumors of the Central Nervous System, vol 5. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-2019-0_8

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  • DOI: https://doi.org/10.1007/978-94-007-2019-0_8

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  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-007-2018-3

  • Online ISBN: 978-94-007-2019-0

  • eBook Packages: MedicineMedicine (R0)

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