Abstract
Bone marrow stromal cells (BMSCs) include cells with multi-directional differentiation potential, such as the mesenchymal stem cells (MSCs). For clinical use, it is important to develop safe and efficient methods of isolating BMSCs from the bone marrow. A new concept is to use a filtering device that selectively traps BMSCs from bone marrow aspirates based on its affinity to the filter material. The cells are then recovered by a retrograde flow in a closed system. This method is more efficient, faster, and easier to use than the density gradient method. Because this method is performed in a closed system without centrifugation, no biologically clean area is required, giving this method a great advantage in clinical applications.
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The authors thank Ms. M. Ueda and Mr. S. Yoshida for reviewing.
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© 2012 Springer Science+Business Media B.V.
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Aoyama, T., Toguchida, J. (2012). Isolation of Bone Marrow Stromal Cells from Bone Marrow by Using a Filtering Device (Method). In: Hayat, M. (eds) Stem Cells and Cancer Stem Cells, Volume 2. Stem Cells and Cancer Stem Cells, vol 2. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-2016-9_1
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DOI: https://doi.org/10.1007/978-94-007-2016-9_1
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