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Optical Detection of Non-amplified Genomic DNA

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Detection of Non-Amplified Genomic DNA

Part of the book series: Soft and Biological Matter ((SOBIMA))

Abstract

Nucleic acid sequences are unique to every living organisms including animals, plants and even bacteria and virus, which provide a practical molecular target for the identification and diagnosis of various diseases. DNA contains heterocyclic rings that has inherent optical absorbance at 260 nm, which is widely used to quantify single and double stranded DNA in biology. However, this simple quantification method could not differentiate sequences; therefore it is not suitable for sequence-specific analyte detection. In addition to a few exceptions such as chiral-related circular dichroism spectra, DNA hybridization does not produce significant changes in optical signals, thus an optical label is generally needed for sequence-specific DNA detection with optical means. During the last two decades, we have witnessed explosive progress in the area of optical DNA detection, especially with the help of simultaneously rapidly developed nanomaterials. In this chapter, we will summarize recent advances in optical DNA detection including colorimetric, fluorescent, luminescent, surface plasmon resonance (SPR) and Raman scattering assays. Challenges and problems remained to be addressed are also discussed.

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  1. Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201800, China

    Di Li & Chunhai Fan

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  1. Di Li
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Correspondence to Chunhai Fan .

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  1. , Department of Chemical Science, University of Catania, Viale Andrea Doria 6, Catania, 95125, Italy

    Giuseppe Spoto

  2. , Faculty of Science, University of Parma, Parma, 43100, Italy

    Roberto Corradini

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Li, D., Fan, C. (2012). Optical Detection of Non-amplified Genomic DNA. In: Spoto, G., Corradini, R. (eds) Detection of Non-Amplified Genomic DNA. Soft and Biological Matter. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-1226-3_6

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