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Engineered Nanostructures for the Ultrasensitive DNA Detection

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Detection of Non-Amplified Genomic DNA

Part of the book series: Soft and Biological Matter ((SOBIMA))

Abstract

Coupled with nanotechnologies, a wide variety of DNA sensing methods have been developed to achieve ultrahigh sensitivity and selectivity without the aid of enzymatic amplification procedures or complicated assay procedures. Structurally engineered nanomaterials have several useful aspects including their unique optical properties depending on size, shape, composition and structural details and electrical properties, which have been translated into various signal transduction modes. However, the most important challenge in DNA detection assay to compete with or complement the polymerase chain reaction (PCR) is matching the sensitivity of PCR, which can detect 10–100 copies in whole sample via various non-enzymatic amplification strategies. Here, we introduce recent advances in engineered nanostructure-based DNA detection methods that show potential for PCR-like sensitivity and can address the existing issues of conventional DNA detection assays. The basic principles, advantages, and limitations of engineered nanostructure-amplified DNA detection methods will be introduced and discussed.

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Authors and Affiliations

  1. Department of Chemistry, Seoul National University, Seoul, 151-747, South Korea

    Dong-Kwon Lim, Amit Kumar & Jwa-Min Nam

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  1. Dong-Kwon Lim
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  2. Amit Kumar
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  3. Jwa-Min Nam
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Correspondence to Jwa-Min Nam .

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Editors and Affiliations

  1. , Department of Chemical Science, University of Catania, Viale Andrea Doria 6, Catania, 95125, Italy

    Giuseppe Spoto

  2. , Faculty of Science, University of Parma, Parma, 43100, Italy

    Roberto Corradini

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Lim, DK., Kumar, A., Nam, JM. (2012). Engineered Nanostructures for the Ultrasensitive DNA Detection. In: Spoto, G., Corradini, R. (eds) Detection of Non-Amplified Genomic DNA. Soft and Biological Matter. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-1226-3_3

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