Abstract
This contribution introduces strategies for the sensitive detection of oligonucleotides as bio-analytes binding from solution to a variety of probe architectures assembled at the (Au-) sensor surface. Detection principles based on surface plasmon optics and electrochemical techniques are compared. In particular, cyclic- and square wave voltammetry (SWV) are applied for the read-out of ferrocene redox labels conjugated to streptavidin that binds to the (biotinylated) DNA targets after hybridizing to the interfacial probe matrix of either DNA or peptide nucleic acid (PNA) strands. By employing streptavidin modified with fluorophores the identical sensor architecture can be used for the recording of hybridization reactions by surface plasmon fluorescence spectroscopy (SPFS). The Langmuir isotherms determined by both techniques, i.e., by SWV and SPFS, give virtually identical affinity constants KA, confirming that the mode of detection has no influence on the hybridization reaction. By using semiconducting nanoparticles as luminescence labels that can be tuned in their bandgap energies over a wide range of emission wavelengths surface plasmon fluorescence microscopy allows for the parallel read-out of multiple analyte binding events simultaneously.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Kastner, M.A.: The single electron transistor and artificial atoms. Ann. Phys. 9, 885–894 (2000)
Knoll, W.: Interfaces and thin films as seen by bound electromagnetic waves. Annu. Rev. Phys. Chem. 49, 569–638 (1998)
Kaifer, A.E., Gómez-Kaifer, M.: Supramolecular Electrochemistry. Wiley- VCH, Weinheim (2001)
Liebermann, T., Knoll, W.: Colloids and Surfaces A 171, 115–130 (2000)
Robelek, R., Niu, L., Schmid, E., Knoll, W.: Multiplexed hybridization detection of quantum Dot conjugated DNA sequences using surface Plasmon enhanced fluorescence microscopy and spectrometry. Anal. Chem. 15, 6160–6165 (2004)
Cameron, P.J., Zhong, X., Knoll, W.: Electrochemically controlled surface Plasmon enhanced fluorescence response of surface immobilized CdZnSe quantum dots. J. Phys. Chem. C 113, 6003–6008 (2009)
Knoll, W., Yu, F., Neumann, T., Niu, L., Schmid, E.: Principles and applications of surface-plasmon field- enhanced fluorescence techniques. In: Lakowicz, J.R., Geddes, C.D. (eds.) Topics in Fluorescence Spectroscopy. Vol 8: Radiative Decay Engineering, pp. 305–332. Springer, Berlin/Heidelberg/New York (2005)
Yao, D., Yu, F., Kim, J., Scholz, J., Nielsen, P., Sinner, E.K., Knoll, W.: Surface plasmon field-enhanced fluorescence spectroscopy in PCR product analysis by peptide nucleic acid probes. Nucleic Acids Res. 32, 177–192 (2004)
Liu, J., Tian, S., Tiefenauer, L., Nielsen, P., Knoll, W.: Simultaneously amplified electrochemical and surface plasmon resonance optical detection of DNA hybridization based on ferrocene-streptavidin conjugates. Anal. Chem. 77, 2756–2761 (2005)
Liu, J., Tian, S., Tiefenauer, L., Nielsen, P., Knoll, W.: PNA-DNA hybridization study using labeled-streptavidin by voltammetry and surface plasmon fluorescence spectroscopy. Anal. Chem. 78, 470–476 (2006)
Yu, F., Persson, B., Lofas, S., Knoll, W.: Attomolar sensitivity in bioassays based on surface plasmon fluorescence spectroscopy. J. Am. Chem. Soc. 126, 8902–8903 (2004)
Zizlsperger, M., Knoll, W.: Multispot parallel on-line monitoring of interfacial binding reactions by surface plasmon microscopy. Prog. Colloid Polym. Sci. 109, 244–253 (1998)
Liebermann, T., Knoll, W.: Parallel multispot detection of target hybridization to surface-bound probe oligonucleotides of different base mismatch by surface-plasmon field-enhanced fluorescence microscopy. Langmuir 19, 1567–1572 (2003)
Zhong, X., Xie, R., Zhang, Y., Basché, T., Knoll, W.: High-quality violet- to red-emitting ZnSe/CdSe core/shell nanocrystals. Chem. Mater. 17, 4038–4042 (2005)
Niu, L., Knoll, W.: Electrochemically addressable functionalization and parallel readout of a DNA biosensor array. Anal. Chem. 79, 2695–2702 (2007)
Robelek, R., Stefani, F.D., Knoll, W.: Oligonucleotide hybridization monitored by surface plasmon enhanced fluorescence spectroscopy with bio-conjugated core/shell quantum dots. Influence of luminescence blinking. Phys. Status Solid A 203, 3468–3475 (2006)
Sinner, E.-K., Ritz, S., Wang, Y., Dostalek, J., Jonas, U., Knoll, W.: Molecularly controlled functional architectures at biointerfaces. Mater. Today 13, 46–55 (2010)
Acknowledgments
Partial funding for this project from the Deutsche Forschungsgemeinschaft (DFG, KN 224/13-1 and 13-2, and SFB 625) is gratefully acknowledged. We thank Thomas Basché, Ming-Yong Han, and Shengjun Tian for many helpful discussions.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Knoll, W., Liu, J., Niu, L., Nielsen, P.E., Tiefenauer, L. (2012). Parallel Optical and Electrochemical DNA Detection. In: Spoto, G., Corradini, R. (eds) Detection of Non-Amplified Genomic DNA. Soft and Biological Matter. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-1226-3_10
Download citation
DOI: https://doi.org/10.1007/978-94-007-1226-3_10
Published:
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-007-1225-6
Online ISBN: 978-94-007-1226-3
eBook Packages: Physics and AstronomyPhysics and Astronomy (R0)