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Plant Breeding pp 473–499Cite as

Cytoplasmic-Nuclear Male Sterility: Origin, Evaluation and Utilization

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Abstract

Cytoplasmic-nuclear male sterility (CMS), a potential system for economical hybrid seeds production, results from disharmonious interaction between the cytoplasmic and nuclear genetic factors. Evaluation of CMS system for its utility is determined by many factors. Unstable male sterility increases cost and affects quality of hybrid seed production. Cooler weather during panicle development promotes instability in maize and pigeonpea, high humidity in pearl millet and hot temperature of 42°C and above in sorghum. Effect of genetic background of B-lines on male sterility has been found in case of A1 CMS system in pearl millet and sorghum, and fertility restorer gene expressivity in rice. Maintainer gene frequency in germplasm is another factor to be considered which has bearing on genetic diversification of A-lines. In case of A1 CMS system in sorghum and pearl millet landraces have greater proportion as restorers. Character association influences the commercial viability of a CMS system. Approaches to estimate the diversity of CMS systems to enhance the exploitation of outstanding inbred lines and also to overcome the risk of disease and pest epidemics, include both traditional field evaluation of hybrids for differential patterns of male fertility restoration and RFLP analysis of mitochondrial DNA. Steps involved in CMS utilization in hybrid cultivar breeding including (i) identification of potential CMS sources, (ii) development of seed parents (A lines) exhibiting complete sterility, (iii) maintenance of seed parents by taking appropriate steps to prevent contamination with foreign pollen, (iv) development of restorer parents through identification of promising restorers, genetic improvement of existing restorers and transfer of restorer genes into high yielding inbred lines and (v) hybrid seed production keeping in view the four key elements — isolation distance, off type removal, flowering synchrony between A- and R-lines, and A:R lines ratio are discussed.

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References

  • An and Kumar K., Jain R. P., and Singh S. D. 1983. Downy mildew reactions of pearl millet lines with and without cytoplasmic male sterility. Plant Dis., 67: 663–665.

    Article  Google Scholar 

  • Anonymous. 1998. Hybrid Rice Progress Report 1989–98. ICAR project on promotion of Research and development efforts on hybrids in selected crops — Rice. Directorate of Rice Research, Rajendranagar, Hyderabad, India. 131 pp.

    Google Scholar 

  • Appa Rao S., Mangesha M. H. and Rajagopal Reddy C. 1989. Development of cytoplasmic male-sterile lines of pearl millet from Ghana and Botswana germplasm. Pp 817–823. In: Perspectives in Cytology and Genetics, (eds. ) G. K. Manna and U. Sinha, All India Congress of Cytology and Genetics, Kalyani Publishers, India.

    Google Scholar 

  • Athwal D. S. 1961. Recent developments in the breeding and improvement of bajra (pearl millet) in the Punjab. Madras Agric. J., 48: 18–19 (Abstract).

    Google Scholar 

  • Athwal D. S. and Virmani S. S. 1972. Cytoplasmic male sterility and hybrid breeding in rice. In: Rice Breeding, pp. 615–620. Int. Rice Res. Inst. Manila, Philippines.

    Google Scholar 

  • Burton G. W. 1958. Cytoplasmic male-sterility in pearl millet [Pennisetum glaucum (L. ) R. Br. ]. Agron. J., 50: 230.

    Article  Google Scholar 

  • Brar D. S., Zhu Y. G., Ahmed M. I., Jachuck P. J. and Virmani S. S. 1998. Diversifying the CMS system to improve the sustainability of hybrid rice technology. In: Hybrid Rice Technology, p. 443. Proc. 3rd Intl. Symp. on Hybrid Rice, 14-16 Nov, 1996, Hyderabad, India.

    Google Scholar 

  • Carnhan H. L., Erickson J. R., Tseng S. T. and Rutger J. N. 1972. Outlook for hybrid rice in USA. In: Rice Breeding, pp. 603–607, Int. Rice Res. Inst., Manila, Philippines.

    Google Scholar 

  • Chhabra A. K., Rai K. N., Khairwal I. S., Sivaramakrishnan S. and Hash CT. 1998. Mitochondrial DNA-RFLP analysis distinguishes new CMS sources in pearl millet (Pennisetum glaucum (L. ) R. Br. ). J. Plant Biochem. and Biotech., 7: 85–92.

    Article  CAS  Google Scholar 

  • Dalmacio R. D., Brar D. S., Ishii T., Sitch L. A., Virmani S. S. and Khush G. S. 1995. Identification and transfer of a new cytoplasmic male sterility sources from Oryza perennis into indica rice (Oryza sativa L. ) Euphytica, 82: 221–225.

    Article  Google Scholar 

  • Dalmacio R. D., Brar D. S., Virmani S. S. and Khush G. S. 1996. Male sterile line in rice (Oryza sativa) developed with O. glumaepatula cytoplasm. IRRN., 21: 22–23

    Google Scholar 

  • Devi D. R. and Murthy U. R. 1993. Genotype environment interaction in tapetal development A2 based sorghum genotypes. Sorghum Newsl., 34: 41.

    Google Scholar 

  • Downes R. W. and Marshall D. R. 1971. Low temperature induced male sterility in Sorghum bicolor. Aust. J. Exp. Agric. Anim. Husb., 11: 352–356.

    Article  Google Scholar 

  • Duncan R. R., Bramel-Cox P. J. and Miller F. R. 1991. Contributions of introduced sorghum germplasm to hybrid development in the USA. In: Use of plant introductions in cultivar development. Part 1. (eds. ) Shands H. L. and L. E. Wiesner, Proceedings of a symposium sponsored by Division C-l of the Crop Sci. Society of America in Las Vegas, Nevada, USA, pp. 69–102, Crop Sci. Society of America, Madison, Wisconsin, USA.

    Google Scholar 

  • Duvick D. N. 1965. Cytoplasmic pollen sterility in corn. Adv. Genet., 13: 1–52.

    Article  Google Scholar 

  • Ganesan K. N., Thiyagarajan K. and Rangaswamy M. 1998. Effect of minor genes in restoration fertility in CMS lines of rice. International Rice Res. Notes., 23: 9.

    Google Scholar 

  • Govindraj K. and Virmani S. S. 1988. Genetics of fertility restoration of WA type cytoplasmic male-sterility in rice. Crop Sci., 28: 787–792.

    Article  Google Scholar 

  • Hanna W. W. 1989. Characteristics and stability of a new cytoplasmic-nuclear male-sterile source in pearl millet. Crop Sci., 27: 1457–1459.

    Article  Google Scholar 

  • Hoan N. T., Sarma N. P. and Siddiq E. A. 1997. Identification and characterization of new sources of cytoplasmic male sterility in rice. PL Breed., 116: 547–551.

    Article  Google Scholar 

  • House L. R. 1985. A Guide to Sorghum Breeding. (2nd Edn. ). International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru 502 324, A. P., India. 206 pp.

    Google Scholar 

  • Kishan A. G. and Borikar S. T. 1989a. Genetic relationship between some cytoplasmic male sterility systems in sorghum. Euphytica, 42: 259–269.

    Google Scholar 

  • Kishan A. G. and Borikar S. T. 1989b. Comparative performance of Maldandi vs Milo cytoplasm in sorghum. J. Maharashtra Agric. Univ., 14: 192–195.

    Google Scholar 

  • Kumari S. L., Mahadevappa M. and Kulkarni R. S. 1998. Fertility restoration study in four WA CMS lines of rice. Int. Rice Res. Notes, 23: 9.

    Google Scholar 

  • Li Zhengyon. 1980. Dion type hybrid rice. Yuan People’s Press, China.

    Google Scholar 

  • Li Z. J., Zhang K. T., Oing Y. X. and Xo J. X. 1981. Effect of climate on male sterility in sorghum. Agron. Sinica, 7: 129–133.

    Google Scholar 

  • Lin S. C. and Yuan L. P. 1980. Hybrid rice breeding in China. In: Innovative approaches in Rice Breeding, pp. 35–51, Int. Rice Res. Inst. Manila, Philippines.

    Google Scholar 

  • Lin S. C. and Ming S. K. 1991. Rice varieties and their geneology in China. Shanghai Scientific and Technical Publishers, Shanghai, China.

    Google Scholar 

  • Lin Z. and Zhu Y. 1988. Rice male-sterile cytoplasm and fertility restoration. In: Hybrid Rice. pp. 85–102, Int. Rice Res. Inst. Manila, Philippines.

    Google Scholar 

  • Madhava Menon P. M. 1959. Occurrence of cytoplasmic male-sterility in pearl millet (Pennisetum typhoides Stapf & Hubb. ), Curr. Sci., 28: 165–167.

    Google Scholar 

  • Mao C. X., Virmani S. S. and Ish Kumar. 1998. Technological innovations to lower the costs of hybrid seed production. In: Advances in hybrid rice technology. Proceedings of 3rd International Symposium on Hybrid Rice. November 14-16, 1996 pp. 111–128.

    Google Scholar 

  • Marchais L. and Pernes J. 1985. Genetic divergence between wild and cultivated pearl millets (Pennisetum typhoides). I. Male sterility. Z Pflanzenzuchtg, 95: 103–112.

    Google Scholar 

  • Maruyama K., Araki K. and Keto H. 1991 Thermosensitive genetic male sterility induced by irradiation. In: Rice Genetics II, pp. 227–232, Int. Rice Res. Inst. Manila.

    Google Scholar 

  • Maunder A. B. 1972. Objectives and approaches to grain and forage sorghum improvement in the Americas. In: Sorghum in Seventies, (eds. ) N. Ganga Prasada Rao and L. R. House. Oxford and IBH publishing Co., New Delhi, India. pp. 60–99.

    Google Scholar 

  • Murthy U. R. 1986. Effect of A2 cytoplasm on the inheritance of plant height in temperate × tropical sorghum crosses. Sorghum Newsl., 29: 77.

    Google Scholar 

  • Nagur T. and Menon P. M. 1974. Characterization of male sterility-inducing cytoplasms in sorghum. Sorghum Newsl., 17: 18.

    Google Scholar 

  • Pan Xigan. 1982. Analysis of the effect of cytoplasm on heterosis. Agricultural Science & Technology, Jiangxi.

    Google Scholar 

  • Pradhan S. B. and Jachuck P. J. 1998. Krishna CMS line in the background of four different cytoplasmic sources in rice. Int. Rice. Res. Notes, 23: 6.

    Google Scholar 

  • Pradhan S. B., Ratho S. N. and Jachuck P. J. 1990. New sources of cytoplasmic-genetic male-sterility in cultivated indica rices. Euphytica, 48: 215–218.

    Article  Google Scholar 

  • Rai K. N., Andrews D. J. and Suresh Babu V. 1985. Inbreeding depression in pearl millet composites. Z. Pflanzenzuchtg, 94: 201–207.

    Google Scholar 

  • Rai K. N. and Hash C. T. 1993. A new source of cytoplasmic-nuclear male-sterility in pearl millet. Cereals Program, ICRISAT. Annual Report (1992).

    Google Scholar 

  • Rai K. N. 1995. A new cytoplasmic-nuclear male-sterility system in pearl millet. Plant Breed., 114: 445–447.

    Article  Google Scholar 

  • Rai K. N. and Thakur R. P. 1995. Ergot reaction of pearl millet hybrids affected by fertility restoration and genetic resistance of parental lines. Euphytica, 83: 225–231.

    Article  Google Scholar 

  • Rai K. N. and Thakur R. P. 1996. Smut reaction of pearl millet hybrids affected by fertility restoration and genetic resistance of parental lines. Euphytica, 90: 31–37.

    Google Scholar 

  • Rai K. N., Andrews D. J., Rao A. S., Rajewski J. F. and Du R. H. 1999. Restorer sources of A5 cytoplasmic-nuclear male sterility in Pennisetum germplasm and its implications in pearl millet hybrid breeding. Plant Genetic Resources Newsletter, 1999, 120: 20–24.

    Google Scholar 

  • Reddy Belum V. S. 1992. Varietal improvement: Three-way single cross hybrids. In: Annual Report 1991, pp. 66–67, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru 502 324, Andhra Pradesh, India.

    Google Scholar 

  • Reddy Belum V. S. 1997. Production of sorghum hybrids. In: Training Manual on Development of Cultivars and Seed Production Techniques in Sorghum and Pearl Millet, (eds. ) Singh, Faujdar, K. N. Rai, Belum V. S. Reddy & B. Diwakar. pp. 31–33, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru 502 324, Andhra Pradesh, India.

    Google Scholar 

  • Reddy Belum V. S. and Stenhouse J. W. 1994. Sorghum improvement for the semi-arid tropic region: past, current and future research thrusts in Asia. Punjabrao Krishi Vidyapeeth Res. J., 18: 155–170.

    Google Scholar 

  • Sahadevan P. C. and Namboodri K. M. N. 1963. National outcrossing in rice. Proc. Indian Acad. Sci., Sect. B., 58: 176–185.

    Google Scholar 

  • Sampath S. and Mohanty H. K. 1954. Cytology of semi sterile rice hybrid. Curr. Sci., 23: 182–183.

    Google Scholar 

  • Sarma N. P., Sundaram R. M., Thirumurugan T., Viraktamath B. C., Ramesha M. S., Yashitola J. and Sonti R. 2001. DNA marker technology for purity assessment of rice hybrids and parental stocks. ICAR Science and Technology Newsletter, 7: 1–2.

    Google Scholar 

  • Saxena K. B. 2001a. Heterosis breeding in Pulses — Problems and Prospects, pp. 61–72. In: Pulses for Sustainable Agriculture and Nutritional Security, (eds. ) Ali, Masood, Chaturvedi S. K. and Gurha S. N. Indian Institute of Pulses Research, Kanpur.

    Google Scholar 

  • Saxena K. B. 2001b. Prospects for hybrid pigeopea. Paper presented at National Symposium on Pulses and Oil Seeds on sustainable agriculture. Tamil Nadu Agricultural University, Coimbatore. 29-31 July 2001.

    Google Scholar 

  • Scheifele G. L, Whitehead G. W. and Rowe C. 1970. Increased susceptibility to southern leaf spot (Helminthosporium maydis) in inbred lines and hybrids of maize with Texas male-sterile cytoplasm. Plant Dis. Rep., 54: 501–503.

    Google Scholar 

  • Schertz K. F. and Ritchey J. R. 1977. Cytoplasmic-genetic male-sterility systems in sorghum. Crop Sci., 18: 890–893.

    Article  Google Scholar 

  • Schertz K. F. and Pring D. R. 1982. Cytoplasmic sterility systems in sorghum. In: Proceedings of an International Symposium on Sorghum in the Eighties, (eds. ) Rao, N. G. P. & L. R. House, pp. 373–383, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) center, Patancheru 502 324, Andhra Pradesh, Indi

    Google Scholar 

  • Schertz K. F., Sotomayor-Rios A. and Torraco-Cardona S. 1989. Cytoplasmic-nuclear male-sterility opportunities in breeding and genetics. Proceedings of the Grain Sorghum Research and Utilization Conference, 16: 175–186.

    Google Scholar 

  • Schertz K. F., Sivaramakrishnan S., Hanna W. W., Mullet J., Yi Sun, Murty,U. R., Pring D. R., Rai K. N. and Reddy Belum V. S. 1997. Alternate cytoplasms and apomixis of sorghum and millet. In: Proceedings of the International Conference on Genetic Improvement of Sorghum and Pearl Millet, 22-27 September 1996, Holiday Inn Plaza, Lubbock, Texas, USA. pp. 213–223, Texas Agricultural University

    Google Scholar 

  • Scheuring J. F. and Miller F. R. 1978. Fertility restorers and sterility maintainers to the milo-kafir genetic cytoplasmic male sterility system in the sorghum world collection, Misc. Publ., Texas A&M Agricultural Experiment Station, College Station, Texas, USA, 1367: 47 pp.

    Google Scholar 

  • Senthil N., Ramasamy P. and Khan A. K. F. 1998. Fertility restoration and heterosis involving different cytoplasms in sorghum [Sorghum bicolor (L. ) Moench] hybrids. J. Genet. & Breeding, 52: 339–342.

    Google Scholar 

  • Seth P., Sane A. P., Nath P. and Sane P. V. 1996. Molecular characterization of mitochondrial genomes of rice lines containing wild abortive (WA) male-sterile and fertile cytoplasms. J. Plant. Biochemistry & Biotechnology, 5: 75–82.

    Article  CAS  Google Scholar 

  • Sharma H. C., Vidyasagar P., Abraham C. V. and Nwanze K. F. 1994. Effect of cytoplasmic male-sterility in sorghum on host plant interaction with sorghum midge, Contarinia sorghicola. Euphytica, 74: 35–39.

    Google Scholar 

  • Shen Xiaobang. 1982. Preliminary study on the genetic effect of rice sterile cytoplasm. I. The effect of sterile cytoplasms on the agronomic characters of hybrid rice. J. Huazhong Agric. Coll., 3.

    Google Scholar 

  • Shinjyo C. and Omura T. 1966. Cytoplasmic male sterility in cultivated rice, Oryza sativa L. I. Fertiles of F1, F2, and offsprings obtained from their mutual reciprocal backcrosses and segregation of completely male sterile plants. Jpn. J. Breed., 16: 179–180.

    Google Scholar 

  • Shinjyo C. 1969. Cytoplasmic-genetic male-sterility in cultivated rice Oryza Sativa L. II. The inheritance of male sterility. Jpn. J. Genet., 44: 149–156.

    Article  Google Scholar 

  • Siddiq M. A., Madrap I. A. and Ambekar S. S. 1993. Heterosis studies in sorghum. In: The Indian Journal of Genetics & Plant Breeding. Abstract of papers, pp. 123–124. National symposium on Plant Breeding Strategies for India 2000 A. D. and Beyond, Dec 25-27, 1993.

    Google Scholar 

  • Sivaramakrishnan S., Seetha K. and Reddy Belum V. S. 1997. Characterization of the A4 cytoplasmic male-sterile lines of sorghum using RFLP of mt DNA. Euphytica, 93: 301–305.

    Article  CAS  Google Scholar 

  • Stephens J. C. and Holl and P. F. 1954. Cytoplasmic male sterility for hybrid sorghum seed production. Agron. J., 46: 20–23.

    Article  Google Scholar 

  • Virk D. S. 1988. Biometrical analysis in pearl millet: a review. Crop Improv., 15: 1–9.

    Google Scholar 

  • Virmani S. S., Govind Raj K., Casal C., Dalmacio R. D. and Aurin P. A. 1986. Current knowledge and outlook on cytoplasmic-genetic male-sterility and fertility restoration in rice. In: Rice Genet., pp. 633–647, Int. Rice Res. Inst., Manila, Philippines.

    Google Scholar 

  • Virmani S. S. and Shinjyo C. 1988. Current status of analysis and symbols for male sterile cytoplasm and fertility restoring genes. Rice Genet. Newsl., 5: 9–15.

    Google Scholar 

  • Virmani S. S. and Wan B. H. 1988. Development of CMS lines in hybrid rice breeding. In: Hybrid rice. pp. 103–114. Int. Rice Res. Inst. Manila, Philippines.

    Google Scholar 

  • Virmani S. S. 1994. Prospects of hybrid rice in the tropics and sub-tropics. In: Hybrid Rice Technology — New Developments and Future Prospects. pp. 7–20. Int. Rice Res. Inst, Los Banos, Philippines.

    Google Scholar 

  • Virmani S. S. 1996. Hybrid Rice. Adv. Agron., 57: 377–463.

    Google Scholar 

  • Virmani S. S., Viraktamath B. C., Casal C. L., Toledo R. S., Lopez M. T. and Manalo J. O. 1997. Hybrid Rice Breeding Manual. Int. Rice Res. Inst., Las Banos, Laguna, Philippines. P. 151.

    Google Scholar 

  • Webster O. J. and Singh S. P. 1964. Breeding behavior and histological structure of a nondehiscent anther character in Sorghum vulgare. Crop Sci., 4: 656–658.

    Google Scholar 

  • Yadav O. P., Manga V. K. and Gupta G. K. 1993. Influence of A1 cytoplasmic substitution on the downy mildew incidence in pearl millet. Theor. Appl. Genet., 87: 558–560.

    Article  Google Scholar 

  • Yang Rencui. 1980. Effect of sterile cytoplasm of wild abortive rice on F1 generation J. Fujian Agric. Coll., 12: 14–21.

    Google Scholar 

  • Yuan L. P. 1977. The execution theory of developing hybrid rice. Zhonggue Nongye Kexue. Chinese Agric. Sci., 1: 27–31.

    Google Scholar 

  • Yuan L. P. 1985. A concise course in hybrid rice. Human Technological press, China. p. 168.

    Google Scholar 

  • Yuan L. P. and Virmani S. S. 1988. Organization of hybrid rice breeding program. In: Hybrid Rice. pp. 33–37. Int. Rice Res. Inst., Manila, Philippines.

    Google Scholar 

  • Yuan L. P, Virmani S. S. and Mao C. X. 1989. Hybrid Rice — Achievements and future outlook. In: Progress in Irrigated Rice Research. pp. 219–235, Int. Rice Res. Inst., Manila, Philippines.

    Google Scholar 

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Authors and Affiliations

  1. International Crops Research Inst. for the Semi-Arid Tropics, Patancheru, 502 324, India

    B. V. S. Reddy, K. N. Rai & K. B. Saxena

  2. Directorate of Rice Research, Hyderabad, 500 030, India

    N. P. Sarma

  3. Hybrid Rice International Ltd., Hyderabad, 500 008, India

    Ish Kumar

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  1. B. V. S. Reddy
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  4. Ish Kumar
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  5. K. B. Saxena
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H K Jain M C Kharkwal

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Reddy, B.V.S., Rai, K.N., Sarma, N.P., Kumar, I., Saxena, K.B. (2004). Cytoplasmic-Nuclear Male Sterility: Origin, Evaluation and Utilization. In: Jain, H.K., Kharkwal, M.C. (eds) Plant Breeding. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-1040-5_20

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