Abstract
Three species of marine brown macroalgae (seaweeds), Myagropsis myagroides, Sargassum henslowianum and S. siliquastrum collected from Tung Ping Chau, Hong Kong were studied for their curative effects on hepatotoxicity caused by carbon tetrachloride (CCI4) in male Sprague-Dawley rats. A single suitable oral dose of 1.25 ml kg”1 of 20% CCI4 was used as a model hepatotoxin to produce significantly elevated levels of serum glutamic pyruvic transaminase (SGPT) and glutamic oxaloacetic transaminase (SGOT). Gavage oral administration of 300 mg kg”1 of methanol crude extract from S. siliquastrum 6 h post-treatment of CCI4 significantly reduced the CCI4-induced acute elevation in the levels of SGPT and SGOT in rats. Similar results, though at a less effective level, were achieved for extracts from S. henslowianum and M. Myagroides. These results indicate that these seaweeds may contain some active principles in their methanol extracts which acted as an antidote against the hepatotoxicity induced by CCI4. Further investigation is necessary to clarify and characterize the active component(s) in the extracts.
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Refrences
Borzelleca, J. K, T. M. O’ Hara, C. Gennings, R. H. Granger, M. A. Sheppard & L. W. Condie. Jr., 1990. Interactions of water contaminants. I. Plasma enzyme activity and response surface methodology following gavage administration of CCI4 and CHCI3 or TCE singly and in combination in the rat. Fundam. Appl. Toxicol. 14: 477–490.
Chenoweth, M. B. & C. L. Hake, 1962. The smaller halogenated aliphatic hydrocarbons. Ann. Rev. Pharmac. 2: 363–398.
Gilani, A. H. & K. H. Janbazz, 1995a. Studies on protective effect of Cyperus scariosus extract on acetaminophen and CCI4-induced hepatotoxicity. Phytotherapy Res. 9: 489–494.
Gilani, A. H. & K. H. Janbazz, 1995b. Preventive and curative effects of Artemisia absinthium on acetaminophen and CCI4-induced hepatotoxicity. Gen. Pharmac. 26: 309–315.
Gilani, A. H. & K. H. Janbazz, 1995c. Preventive and curative effects of Berberis aristata fruit extract on paracetamol and CCI4-induced hepatotoxicity. Gen. Pharmac. 26: 627–631.
Harada, H., T. Noro & Y. Kamei, 1997. Selective antitumor activity in vitro from marine algae from Japan coasts. Biol. Pharm. Bull. 20: 541–546.
Harris, R. N., J. H. Ratnayake, V. F. Garry & M. W. Anders, 1982. Interactive hepatotoxicity of CHCI3 and CCI4 Toxicol. Appl. Pharmacol. 63: 281–291.
Klaassen, C. D. & G. L. Plaa, 1969. Comparison of the biochemical alterations elicited in livers from rats treated with CCI4, CHCI3, 1,1,2-trichloroethane and 1,1,1-trichloroethane. Biochem. Pharmacol. 18: 2019–2027.
Lee, J. H., J. C. Park & J. S. Choi, 1996. The antioxidant activity of Ecklonia stolonifera. Arch. Pharm. Res. 19: 223–227.
Ooi, V. E. C, 1996. Hepatoprotective effect of some edible mushrooms. Phytotherapy Res. 10: 536–538.
Packer, J. E., T. F. Slater & R. L. Wilson, 1978. Reaction of the CCI4-related peroxy free radical (CCI3OO) with amino acids: pulse radiolysis evidence. Life Sei. 23: 2617–2620.
Plaa, G. L. & W. R. Hewitt, 1982. Quantitative evaluation of indices of hepatotoxicity. In Plaa, G. L. & W. R. Hewitt (eds), Toxicology of the Liver. Raven Press, New York: 103–120.
Recknagel, R. O., 1967. Carbon tetrachloride hepatotoxicity. Pharmacol. Rev. 19: 145–208.
Reitman, S. & S. Frankel, 1957. A colorimetric method for the determination of serum oxaloacetic and glutamic pyruvic transaminases. Am. J. Clin. Pathol. 28: 56–63.
Sallie, R., J. M. Tredgeri & R. William, 1991. Drugs and the liver. Biopharmaceut. Drug Dispos. 12: 251–259.
Slater, T. F., 1966. Necrogenic action of carbon tetrachloride in the rat: a speculative mechanism based on activation. Nature (London) 209: 36–40.
Slater, T. F. 1984. Free-radical mechanisms in tissue injury. Biochem. J. 222: 1–15.
Van de Straat, R., J. Van de Vries, A. J. J. Debets & N. E. Vermueulein, 1987. The mechanism of paracetamol-induced hepatotoxicity by 3,5-dialkyl substitution: the role of glutathione depletion and oxidative stress. Biochem. Pharmac. 36: 2065–2071.
Walker, R. M., W. J. Racz & T. F. Mcelligott, 1980. Acetaminopheninduced hepatotoxicity in mice. Lab. Invest. 42: 181–189.
Wang, D. H., K. Ishii, L. X. Zhen & K. Taketa, 1996. Enhanced liver injury in acatalasemic mice following exposure to carbon tetrachloride. Arch. Toxicol. 70: 189–194.
Wong, C. K., 1999. Protective Effects of Seaweeds Against Liver Injury Caused by Carbon Tetrachloride and Trichloroethylene in Rats. M.Phil. Thesis, The Chinese University of Hong Kong, Hong Kong.
Wong, C. K., V. E. C. Ooi & P. O. Ang. Jr., 2000. Protective effects of seaweeds against liver injury caused by carbon tetrachloride in rats. Chemosphere 41: 173–176.
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Wong, CK., Ooi, V.E.C., Ang, P.O. (2004). Hepatoprotective effect of seaweeds’ methanol extract against carbon tetrachloride-induced poisoning in rats. In: Ang, P.O. (eds) Asian Pacific Phycology in the 21st Century: Prospects and Challenges. Developments in Hydrobiology, vol 173. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-0944-7_35
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DOI: https://doi.org/10.1007/978-94-007-0944-7_35
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