Abstract
An improved method for the preparation of chromosomes from the male gametophyte of the alga Laminaria japonica Aresch. was described. The male gametophyte was pretreated with pDB (p-dichlorobenzene) and 8-hydroxyquinoline in order to clear cell wall and soften cytoplasm. The samples were treated by mordant iron alum [FeNH4 (SO4)2-6H2O] followed by staining with haemotoxylin. Well-spread and highly stained chromosomes were observed without precipitation. The chromosome number of male gametophyte of L. japonica was estimated to be 31.
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Zhou, L.R., Dai, J.X., Shen, S.D. (2004). An improved chromosome preparation from male gametophyte of Laminaria japonica (Heterokontophyta). In: Ang, P.O. (eds) Asian Pacific Phycology in the 21st Century: Prospects and Challenges. Developments in Hydrobiology, vol 173. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-0944-7_18
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DOI: https://doi.org/10.1007/978-94-007-0944-7_18
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