Abstract
Two high monospore-producing pigmentation mutants were obtained by treatment with MNNG in Porphyra yezoensis Ueda. The mutants produced many monospores in young gametophytic blades (1 month old) and old large blades (3 months old). Monospore production of the mutants was affected by the culture conditions. The higher the temperature and/or the light intensity, the more the number of monospores released. When the conchospores and monospores of the mutants were in a monoculture at 15 and 20 °C, they did not develop into large blades because their germlings repeatedly released many monospores. However, they developed into large blades when they were in a co-culture with the large gametophytic blades of the wild-type or other pigmentation mutants. One high monospore-producing red mutant (rm-1) was genetically characterized by crossing with the wild-type which does not easily release monospores. In the F1 gametophytic blades from heterozygous conchocelis produced in the crosses, there were unsectored blades (2 types) and sectored blades (6 types) consisting of 2, 3 or 4 sectors having both parental color phenotypes. Sectors of both parental colors appeared in the F1 sectored blades in the proportion 1W:1.O3R, indicating that the mutant rm-1 has a mutation for its color phenotype. In the 4-week-old F1 blades, high monospore production occurred only in the unsectored red blades and the red sectors of the sectored blades. These results indicate that the tendency of high monospore production is associated with the color phenotype of the mutant, suggesting that high monospore production of the mutant rm-1 is controlled by gene(s), which is closely linked with the gene for the mutant color.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Refrences
Aruga, Y. & A. Miura, 1984. In vivo absorption spectra and pigment contents of the two types of color mutants of Porphyra. Jap. J. Phycol. 32: 243–250.
Chen, M. Q., B. F. Zheng & G. Z. Ren, 1985. The influence of temperature on the adherence of the conchospores and the monospores of Porphyra yezoensis Ueda. Trans. Oceanol. Limnol. 3: 66–69 [in Chinese with English abstract].
Kato, M. & Y. Aruga, 1984. Comparative studies on the growth and photosynthesis of the pigmentation mutants of Porphyra yezoensis in laboratory culture. Jap. J. Phycol. 32: 333–347.
Li, S. Y., 1984. The ecological characteristics of monospores of Porphyra yezoensis Ueda and their use in cultivation. Hydrobiologia 116/117:255–258.
Li, S. Y & G. F. Cui, 1980. An observation on the growth and development of sporelings from conchospores and monospores of Porphyra yezoensis. Chin. J. Oceanol. Limnol. 11: 370–374 [in Chinese with English abstract].
Ohme, M., Y. Kunifuji & A. Miura, 1986. Cross experiments of the color mutants in Porphyra yezoensis Ueda. Jap. J. Phycol. 34: 101–106.
Yan, X. H., 1997. Induction, isolation and characterization of pigmentation mutants in Porphyra yezoensis Ueda (Bangiales, Rhodophyta). Ph. D thesis, Tokyo University of Fisheries, Tokyo, Japan. 101 pp+ 42 Tables + 217 Figures.
Yan, X. H. & Y Aruga, 1996. Induction and isolation of pigmentation mutants by treatment of Porphyra yezoensis Ueda (Rhodophyta) with NNG. First Asian Pacific Phycol. Forum, 22-25 July 1996, Sydney, Australia, p 34.
Yan, X. H. & Y Aruga, 1997a. Induction of pigmentation mutants by treatment of monospore germlings with NNG in Porphyra yezoensis Ueda (Bangiales, Rhodophyta). Algae 12: 39–52.
Yan, X. H. & Y Aruga, 1997b. Induction and isolation of pigmentation mutants by treatment of Porphyra yezoensis Ueda (Rhodophyta) with NNG. Phycologia 36: 128.
Yan, X. H. & Y Aruga, 1998. Unstable pigmentation mutants obtained by NNG treatment in Porphyra yezoensis Ueda (Bangiales, Rhodophyta). Jpn. J. Phycol. 46: 89.
Yan, X. H. & Y Aruga, 2000. Genetic analysis of artificial pigmentation mutants in Porphyra yezoensis Ueda (Bangiales, Rhodophyta). Phycol. Res. 48: 177–187.
Yan, X. H., Y Fujita & Y Aruga, 1999. Effect of culture conditions on color segregation in F1 foliose thalli from heterozygous conchocelis in cross-experiments of Porphyra yezoensis Ueda. Jpn. J. Phycol. 47: 101.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2004 Springer Science+Business Media Dordrecht
About this paper
Cite this paper
Yan, XH., Fujita, Y., Aruga, Y. (2004). High monospore-producing mutants obtained by treatment with MNNG in Porphyra yezoensis Ueda (Bangiales, Rhodophyta). In: Ang, P.O. (eds) Asian Pacific Phycology in the 21st Century: Prospects and Challenges. Developments in Hydrobiology, vol 173. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-0944-7_17
Download citation
DOI: https://doi.org/10.1007/978-94-007-0944-7_17
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-3748-8
Online ISBN: 978-94-007-0944-7
eBook Packages: Springer Book Archive