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Deciphering Biofilm Structure and Reactivity by Multiscale Time-Resolved Fluorescence Analysis

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Bacterial Adhesion

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 715))

Abstract

In natural, industrial and medical environments, microorganisms mainly live as structured and organised matrix-encased communities known as biofilms. In these communities, microorganisms demonstrate coordinated behaviour and are able to perform specific functions such as dramatic resistance to antimicrobials, which potentially lead to major public health and industrial problems. It is now recognised that the appearance of such specific biofilm functions is intimately related to the three-dimensional organisation of the biological edifice, and results from multifactorial processes. During the last decade, the emergence of innovative optical microscopy techniques such as confocal laser scanning microscopy in combination with fluorescent labelling has radically transformed imaging in biofilm research, giving the possibility to investigate non-invasively the dynamic mechanisms of formation and reactivity of these biostructures. In this chapter, we discuss the contribution of fluorescence analysis and imaging to the study at different timescales of various processes: biofilm development (hours to days), antimicrobial reactivity within the three-dimensional structure (minutes to hours) or molecular diffusion/reaction phenomena (pico- to milliseconds).

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Acknowledgements

Funding for our work from the French “Pole de Compétitivité Ile-de-France MEDICEN” is greatly appreciated. AES-Chemunex is warmly acknowledged for providing ChemChrom V6 fluorophore. We thank the “department of Essonne” for financial support of the confocal microscope (ASTRE n°A02137) and the INRA MIMA2 microscopic facilities for CLSM imaging. O. Habimana, P. Latour-Lambert and P. Piveteau are acknowledged for their contribution to image acquisition.

Author information

Authors and Affiliations

  1. INRA, UMR 1319 MICALIS, Massy, France

    Arnaud Bridier, Ekaterina Tischenko, Jean-Marie Herry & Romain Briandet

  2. AgroParisTech, UMR 1319 MICALIS, Massy, France

    Arnaud Bridier, Ekaterina Tischenko & Florence Dubois-Brissonnet

  3. STERIS, Fontenay-aux-Roses, Paris, France

    Vincent Thomas

  4. Institut des Sciences Moléculaires d’Orsay, Univ Paris-Sud, FRE 3363, Orsay, France

    Samia Daddi-Oubekka, Karine Steenkeste & Marie-Pierre Fontaine-Aupart

  5. CNRS, Orsay, France

    Samia Daddi-Oubekka, Karine Steenkeste & Marie-Pierre Fontaine-Aupart

  6. Institut Curie/CNRS UMR144 PICT-IBiSA, Paris, France

    François Waharte

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  1. Arnaud Bridier
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  2. Ekaterina Tischenko
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  3. Florence Dubois-Brissonnet
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  4. Jean-Marie Herry
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  5. Vincent Thomas
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  6. Samia Daddi-Oubekka
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  7. François Waharte
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  8. Karine Steenkeste
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  9. Marie-Pierre Fontaine-Aupart
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  10. Romain Briandet
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Correspondence to Romain Briandet .

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  1. , Department I, Protein Evolution, Max-Planck Institute for Developmental B, Spemannstr. 35, Tübingen, 72076, Germany

    Dirk Linke

  2. , Institute of Biotechnology, University of Helsinki, Viikinkaari 1, Helsinki, FIN-00014, Finland

    Adrian Goldman

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Bridier, A. et al. (2011). Deciphering Biofilm Structure and Reactivity by Multiscale Time-Resolved Fluorescence Analysis. In: Linke, D., Goldman, A. (eds) Bacterial Adhesion. Advances in Experimental Medicine and Biology, vol 715. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-0940-9_21

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