Phase Plate Electron Microscopy
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An electron microscope enhanced with phase plates has practical advantages, particular for biological electron microscopy. It permits collecting high-contrast images of close-to-life biological structures with cryo-fixation and without harsh sample preparations including staining. Here we describe the state of the art of phase plate electron microscopy. Focuses are given to methodological aspects and biological applications with two dimensional and three-dimensional imaging.
KeywordsElectron microscopy Tomography Phase plate Phase contrast Thin-film Zernike phase contrast Hilbert differential contrast Cryo-fixation
We owe the development and biological applications of phase contrast TEM with phase plates to the collaborators as follows. Development: Radostin Danev, Shozo Sugitani, Hiroshi Okawara, Toshiyuki Itoh, Toshikazu Honda, Toshiaki Suzuki, Yoshihiro Arai, Fumio Hosokawa, Sohei Motoki, Rasmus Schroeder and Michael Marko. Applications: Yasuko Kaneko, Koji Nitta, Hitoshi Nakamoto, Nobutaru Usuda, Kimie Atsuzawa, Ayami Nakazawa, Kiyokazu Kametani, Masashi Yamaguchi and Mitsutoshi Setou. This work was supported in part by a Grant-in-aid for Creative Scientific Research from MEXT, Japan and by Core Research for Evolutional Science and Technology (CREST) from JST, Japan.
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