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Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009 pp 177–187Cite as

Strategies for Pancreatic Differentiation of Pluripotent Stem Cells

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Part of the book series: ESACT Proceedings ((ESACT,volume 5))

Abstract

The transplantation of insulin-producing cells is a promising option for the treatment of patients suffering from diabetes. Because the amount of transplantable surrogate islets is limited, pluripotent stem cells characterized by self-renewal and differentiation capacity offer unique alternatives for regenerative medicine. Over the last years, embryonic stem (ES) cells isolated from early embryos of mouse and human origin have been used for in vitro differentiation into the endoderm and pancreatic lineages. The application of various differentiation strategies resulted in the formation of insulin-producing cells, but only partially, ES-derived insulin-secreting clusters were able to normalize the blood glucose level of diabetic mice. Recently, induced pluripotent stem (iPS) cells have been established by reprogramming of adult cells via the transfer of pluripotency-associated genes. These iPS cells were also capable to differentiate into insulin-positive cells. Here, we present a short overview about the strategies established for the generation of insulin-producing cells from murine and human pluripotent ES and iPS cells. The data demonstrate the achievements that have been obtained with respect to the production of islet-like cells, but also underline that future efforts are needed to improve their functional status and to translate laboratory data into clinical application.

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Authors and Affiliations

  1. Department of Anatomy and Cell Biology, Martin Luther University Halle-Wittenberg, D-06097, Halle/S, Germany

    Insa S. Schroeder

  2. Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), D-06466, Gatersleben, Germany

    Anna Daniel-Wojcik

  3. In Vitro Differentiation Group, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), D-06466, Gatersleben, Germany

    Anna M. Wobus

Authors
  1. Insa S. Schroeder
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  2. Anna Daniel-Wojcik
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  3. Anna M. Wobus
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Corresponding author

Correspondence to Anna M. Wobus .

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Editors and Affiliations

  1. , University College Dublin, National Institute of Bioprocessing Rese, Engineering Building, Belfield, Dublin, Ireland

    Nigel Jenkins

  2. , National Institute for Cellular Biotechn, Dublin City University, Dublin, Ireland

    Niall Barron

  3. Tecnologica (IBET), ITQB, Instituto de Biologia Experimental e, Oeiras, Portugal

    Paula Alves

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Schroeder, I.S., Daniel-Wojcik, A., Wobus, A.M. (2012). Strategies for Pancreatic Differentiation of Pluripotent Stem Cells. In: Jenkins, N., Barron, N., Alves, P. (eds) Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009. ESACT Proceedings, vol 5. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-0884-6_28

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