Multiplexed Preparation of Biological Samples for Mass Spectrometry Using Gel Electrophoresis
In spite of the perception that gel electrophoresis has limited compatibility with modern mass spectrometry, polyacrylamide gel electrophoresis remain the gold standard for high resolution protein separation. Recent work has sought to capitalize on both the advantages of gel electrophoresis and mass spectrometry as a tool for protein separation and analysis, combining the two technologies into integrated workflows that have unique capabilities. This chapter discusses recent advances of this technology and applications for its use in mass spectrometry assays.
KeywordsElectrophoresis Fractionation Proteomics Quantitation Sample preparation
- Lee, J.E., Kellie, J.F., Tran, J.C., Tipton, J.D., Catherman, A.D., Thomas, H.M., Ahlf, D.R., Durbin, K.R., Vellaichamy, A., Ntai, I., et al. (2009). A robust two-dimensional separation for top-down tandem mass spectrometry of the low-mass proteome. J Am Soc Mass Spectrom 20, 2183–2191.CrossRefGoogle Scholar
- Piersma, S.R., Fiedler, U., Span, S., Lingnau, A., Pham, T.V., Hoffmann, S., Kubbutat, M.H., and Jimenez, C.R. (2010). Workflow comparison for label-free, quantitative secretome proteomics for cancer biomarker discovery: method evaluation, differential analysis, and verification in serum. J Proteome Res 9, 1913–1922.CrossRefGoogle Scholar
- Tran, J.C. (2008). Development of Effective Proteomic Separations for Biological Analysis Using Mass Spectrometry. Thesis (Ph.D) – Dalhousie University, Halifax, Canada. In Chemistry (Halifax, NS, Dalhousie University), p. 177.Google Scholar
- Tran, J.C., and Doucette, A.A. (2009). Multiplexed Size Seperation of Intact Proteins in Solution Phase for Mass Spectrometry. Anal Chem 81, pp 6201–6209.Google Scholar