Multiplexed Preparation of Biological Samples for Mass Spectrometry Using Gel Electrophoresis

  • Jeremy L. NorrisEmail author
  • Alan A. Doucette


In spite of the perception that gel electrophoresis has limited compatibility with modern mass spectrometry, polyacrylamide gel electrophoresis remain the gold standard for high resolution protein separation. Recent work has sought to capitalize on both the advantages of gel electrophoresis and mass spectrometry as a tool for protein separation and analysis, combining the two technologies into integrated workflows that have unique capabilities. This chapter discusses recent advances of this technology and applications for its use in mass spectrometry assays.


Electrophoresis Fractionation Proteomics Quantitation Sample preparation 


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.Protein Discovery, Inc.KnoxvilleUSA
  2. 2.Dalhousie UniversityHalifaxCanada

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