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UV MALDI for DNA Analysis and the Developments in Sample Preparation Methods

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Sample Preparation in Biological Mass Spectrometry

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Abstract

Matrix Assisted Laser Desorption-Ionization Mass Spectrometry (MALDI MS) is a powerful technique for the analysis of short DNA fragments and products of their enzymatic conversions, with majority of it’s practical applications being developed in fields of organic chemistry of oligonucleotides and typing of genetic polymorphisms. While capable of providing unique structural information and demonstrating high speed of data acquisition, results of MALDI analysis strongly depends on the quality and the type of sample preparation method used and the MALDI matrices applied for laser desorption-ionization. This review is focused mainly on these two areas followed by brief description of the most recent developments in the DNA genotyping by MALDI MS.

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  1. Institute of Physical-Chemical Medicine, Moscow, Russian Federation

    Igor P. Smirnov

  2. A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, Russian Federation

    Igor P. Smirnov

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  1. , Department of Genetics and Complex Disea, Harvard School of Public Health, Harvard, Huntington Ave., SPH-1 665, Boston, 02115, Massachusetts, USA

    Alexander R. Ivanov

  2. , Research and Development, Pressure BioSciences, Inc., Norfolk Ave. 14, South Easton, 02375, Massachusetts, USA

    Alexander V. Lazarev

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Smirnov, I.P. (2011). UV MALDI for DNA Analysis and the Developments in Sample Preparation Methods. In: Ivanov, A., Lazarev, A. (eds) Sample Preparation in Biological Mass Spectrometry. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-0828-0_37

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