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MALDI Imaging Mass Spectrometry for Investigating the Brain

  • Isabelle Fournier
  • Céline Mériaux
  • Maxence Wisztorski
  • Randeep Rakwal
  • Michel SalzetEmail author
Chapter

Abstract

Dynamic properties of the nervous system can now be investigated through mass spectrometry technologies. Generally, the application of these powerful techniques requires the destruction of the specimen under study/examination, but recent technological advances have made it possible to directly analyze tissue sections and perform 2-D or 3-D molecular ions mapping. We review the increasing application of matrix-assisted laser desorption/ionization (MALDI) imaging to the analysis of molecular distributions of proteins and peptides in nervous tissues of both invertebrates and vertebrates, focusing in particular on recent studies of neurodegenerative diseases, and early efforts to implement assays of neuronal development.

Keywords

Invertebrates MALDI imaging Neurodegenerative diseases Peptides Vertebrates 

Notes

Acknowledgements

Supported by grants from Centre National de la Recherche Scientifique (CNRS), Ministère de L’Education Nationale, de L’Enseignement Supérieur et de la Recherche, Agence Nationale de la Recherche (ANR to IF) and Genoscope (to MS).

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Isabelle Fournier
    • 1
  • Céline Mériaux
    • 2
  • Maxence Wisztorski
    • 1
  • Randeep Rakwal
    • 3
    • 4
    • 5
  • Michel Salzet
    • 1
    Email author
  1. 1.MALDI Imaging Team, Laboratoire de Spectrométrie de Masse Biologique Fondamentale et Appliquée (FABMS), EA 4550Université Nord de FranceVilleneuve d’Ascq CedexFrance
  2. 2.CNRS, MALDI Imaging Team, Laboratoire de Neuroimmunologie et Neurochimie EvolutivesUniversité Nord de FranceVilleneuve d’Ascq CedexFrance
  3. 3.Laboratory of Neuroscience, Department of BiologyToho UniversityFunabashi, ChibaJapan
  4. 4.Research Laboratory for Biotechnology and Biochemistry (RLABB)KathmanduNepal
  5. 5.School of Medicine, Showa UniversityTokyoJapan

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