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MALDI Imaging Mass Spectrometry for Investigating the Brain

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Sample Preparation in Biological Mass Spectrometry

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Abstract

Dynamic properties of the nervous system can now be investigated through mass spectrometry technologies. Generally, the application of these powerful techniques requires the destruction of the specimen under study/examination, but recent technological advances have made it possible to directly analyze tissue sections and perform 2-D or 3-D molecular ions mapping. We review the increasing application of matrix-assisted laser desorption/ionization (MALDI) imaging to the analysis of molecular distributions of proteins and peptides in nervous tissues of both invertebrates and vertebrates, focusing in particular on recent studies of neurodegenerative diseases, and early efforts to implement assays of neuronal development.

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Acknowledgements

Supported by grants from Centre National de la Recherche Scientifique (CNRS), Ministère de L’Education Nationale, de L’Enseignement Supérieur et de la Recherche, Agence Nationale de la Recherche (ANR to IF) and Genoscope (to MS).

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Authors and Affiliations

  1. MALDI Imaging Team, Laboratoire de Spectrométrie de Masse Biologique Fondamentale et Appliquée (FABMS), EA 4550, Université Nord de France, F-59655, Villeneuve d’Ascq Cedex, France

    Isabelle Fournier, Maxence Wisztorski & Michel Salzet

  2. CNRS, MALDI Imaging Team, Laboratoire de Neuroimmunologie et Neurochimie Evolutives, Université Nord de France, Villeneuve d’Ascq Cedex, France

    Céline Mériaux

  3. Laboratory of Neuroscience, Department of Biology, Toho University, Funabashi, Chiba, Japan

    Randeep Rakwal

  4. Research Laboratory for Biotechnology and Biochemistry (RLABB), Kathmandu, Nepal

    Randeep Rakwal

  5. School of Medicine, Showa University, Tokyo, Japan

    Randeep Rakwal

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  1. Isabelle Fournier
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  2. Céline Mériaux
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  3. Maxence Wisztorski
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  4. Randeep Rakwal
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  5. Michel Salzet
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Correspondence to Michel Salzet .

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Editors and Affiliations

  1. , Department of Genetics and Complex Disea, Harvard School of Public Health, Harvard, Huntington Ave., SPH-1 665, Boston, 02115, Massachusetts, USA

    Alexander R. Ivanov

  2. , Research and Development, Pressure BioSciences, Inc., Norfolk Ave. 14, South Easton, 02375, Massachusetts, USA

    Alexander V. Lazarev

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Fournier, I., Mériaux, C., Wisztorski, M., Rakwal, R., Salzet, M. (2011). MALDI Imaging Mass Spectrometry for Investigating the Brain. In: Ivanov, A., Lazarev, A. (eds) Sample Preparation in Biological Mass Spectrometry. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-0828-0_36

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