Analysis of Glycopeptides Using Porous Graphite Chromatography and LTQ Orbitrap XL ETD Hybrid MS

  • Terry Zhang
  • Rosa Viner
  • Zhiqi Hao
  • Vlad ZabrouskovEmail author


In this study, several glycoproteins: bovine α1-acid glycoprotein, fetuin, and human α1-acid glycoprotein, were analyzed using nanoLC-MS/MS. The performance of three different stationary phases – C8, C18, and porous graphite – was systematically evaluated and optimized for glycopeptide separation. A porous graphite column was chosen as the optimum stationary phase for glycoprotein analysis. An LTQ Orbitrap XL hybrid mass spectrometer equipped with electron transfer dissociation (ETD) was used to acquire, within a single analysis, high-resolution, high-mass-accuracy full MS spectra, collision-induced dissociation (CID) MS/MS spectra for glycan composition analysis, and ETD MS/MS spectra for peptide structure information. As a result, characterization of glycopeptides was achieved within a single LC-MS/MS run.


ETD Glycoprotein N-glycosylation LTQ Orbitrap XL ETD Porous graphite 



We thank David Fisher at Thermo Fisher Scientific for editing and proof reading of this manuscript.


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Terry Zhang
    • 1
  • Rosa Viner
    • 1
  • Zhiqi Hao
    • 1
  • Vlad Zabrouskov
    • 1
    Email author
  1. 1.Thermo Fisher ScientificSan JoseUSA

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