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Sample Handling of Body Fluids for Proteomics

  • Joao A. PauloEmail author
  • Ali R. Vaezzadeh
  • Darwin L. Conwell
  • Richard S. Lee
  • Hanno Steen
Chapter

Abstract

This chapter provides an overview of different approaches that can be used for sample preparation of body fluids for proteomics. Sample collection, protein extraction, protease inhibitor supplementation, sample storage, and abundant protein depletion are presented here in the context of various human body fluids. We emphasize that the particular set of techniques chosen for such investigations tightly correlates with the fluid to be analyzed, as no consensus methods are appropriate for all body fluids. However, we stress the need for standardized methods for the individual body fluids which is paramount in obtaining reproducible and robust data when analyzing human body fluids. In addition, we provide examples of optimized sample handling techniques using a systemic (urine) and a proximal body fluid (pancreatic fluid).

Keywords

Body fluids Biomarkers Sample handling Urine Pancreas Pancreatitis Kidney 

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Joao A. Paulo
    • 1
    • 2
    • 3
    Email author
  • Ali R. Vaezzadeh
    • 4
  • Darwin L. Conwell
    • 1
    • 2
  • Richard S. Lee
    • 4
  • Hanno Steen
    • 3
    • 5
  1. 1.Division of Gastroenterology, Hepatology and EndoscopyCenter for Pancreatic Disease, Brigham and Women’s HospitalBostonUSA
  2. 2.Department of MedicineHarvard Medical SchoolBostonUSA
  3. 3.Department of PathologyChildren’s Hospital Boston, Harvard Medical SchoolBostonUSA
  4. 4.Department of UrologyHarvard Medical School, Children’s Hospital BostonBostonUSA
  5. 5.Proteomics Center at Children’s Hospital BostonBostonUSA

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